摘要
试验旨在研究IGF-I基因调控绵羊生长发育的表达模式和其SNPs位点与生长性状的相关性，采用qRT—PCR技术检测绵羊的大脑、肌肉、皮肤、肝脏和心脏组织的IGF-I基因在15、60、105、150、195和240d等6个时间点的变化，利用SSCP技术分析IGF-I基因的SNPs位点与生长性状的相关性。结果表明：5个组织在105d时出现第1个表达拐点，15～105d时肌肉、大脑和肝脏组织的表达降低后再升高，而皮肤和心脏组织在15和60d时的表达无显著差异，105d时才显著或极显著升高；105d后，肝脏组织的表达逐渐降低，肌肉、心脏、大脑和皮肤组织在195d时出现第2个拐点，但肌肉和心脏组织并未引起表达的显著差异，而大脑和皮肤组织在195d时的表达显著高于150和240d，提示IGF-I基因在5个组织的表达具有一定的同步性；IGpI基因的外显子前导区和外显子3的2个SNP位点为中低度多态性，P-1引物扩增的SNP位点与凉山半细毛羊的初生重具有显著的关联性（P〈O．05）；P-2引物扩增的SNP位点与断奶日增重和断奶重具有显著的关联性（P〈0．05）。IGF-I基因在不同的组织中表现出比较一致的表达模式，其SNP位点与绵羊的早期生长性状存在较强的相关性，为凉山半细毛羊的生长调控机理和SNPs辅助选择育种提供理论基础。
This experiment was conducted to study the expression patten of IGF-I gene regula- ting growth and development of sheep and correlation of its SNPs with growth traits in sheep. The IGF-1 gene expression change of IGF-I in brain,tousle,skin,liver and heart were detected at six growth stages（15,60,105,150,195,240 days） by qRT-PCR approach and the correlation of SNPs of IGF- I gene with growth traits were analyzed by SSCP method in Liangshan Semi-wool sheep population. The results showed that the first inflection point of the expression of IGF-I gene in musle, skin, brain, liver and heart tissues was at 105 day and the second inflection pointwas at 195 day without the liver tissue. From 15 to 105 day, the expression of IGF-1 gene in mu- sle, brain and liver tissues decreased at 60 day, and then increased at 105 day, however, it is not significantly different at 15 and 60 day, and increased significantly at 105 day in skin and heart tissues. After 105 day, the expression of IGF- I were degraded in all of the tissues at 150 day, and the expression in liver tissue was decreased gradually from 105 to 240 day, but the expression in skin and brain tissues were higher at 195 day than that at 150 and 240 day, and no difference at among 150, 195 and 240 day in musle and heart tissues. The two SNPs which were detected with 587 Liangshan Semi-wool sheep were moderate polymorphism. The SNP of the P-1 primer affect- ed significantly birth weight（P〈0.05） and the SNP of the P-2 primer affected significantly wea- ning weight and the weaning daily gain（P〈0.05）. The results suggest that the expression of IGF- I in all tissues have the similar expression patterns,and strong correlation between SNPs of IGF-1 and confirmed the early growth traits provide theoretical basis for the growth regulation and can be applied to assisted selective breeding with the SNPs in Liangshan Semi-wool sheep.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2013年第9期1363-1370,共8页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
四川省畜禽育种攻关项目(01NG029-18)
四川省科技厅支撑项目(2012JY0126)
