摘要
为研究 KISS1基因在常年发情的小尾寒羊和季节性发情的草地型藏羊中的表达模式,以及 KISS1基因多态性与绵羊繁殖之间的关系,实验采用 qPCR技术对比分析 KISS1基因在 2个品种绵羊的 10种繁殖相关组织中的表达差异,同时利用 Sequenom MassARRAY SNP 技术对常年发情绵羊(小尾寒羊、策勒黑羊和湖羊)和季节性发情绵羊(滩羊、苏尼特羊和草地型藏羊)KISS1基因 2 个 SNPs 位点多态性进行检测,并与小尾寒羊产羔数进行关联分析。qPCR 结果显示:KISS1基因在小尾寒羊下丘脑、大脑、垂体和甲状腺中的表达量高于草原型藏羊(P<0.05);分型结果表明,g.1317523C>T 位点基因型频率和等位基因频率在常年发情和季节性发情绵羊品种间差异均达到极显著水平(P<0.01);群体遗传学分析表明,g.1317523C>T 位点在 6 个绵羊群体中均表现为中度多态(0.25<PIC<0.5);卡方适合性检验表明,g.1317523C>T 位点在小尾寒羊、滩羊、苏尼特羊和湖羊中均处于哈代温伯格平衡状态(P>0.05),g.1311578G>T 位点在苏尼特羊和湖羊中处于哈代温伯格平衡状态(P>0.05);关联分析表明,这 2个 SNPs位点与小尾寒羊前 3胎产羔数均无显著关联(P>0.05),但 g.1311578G>T 位点 TT 型各胎产羔数均大于 GT 和 GG 型。综上, KISS1基因与绵羊的季节性繁殖密切相关,并且 g.1311578G>T 位点对绵羊产羔性状有潜在调控作用。
In order to study the expression pattern of KISS1 gene in year-round estrous Small Tail Han sheep and seasonal estrous Prairie Tibetan sheep, and explore the relationship between the polymorphism and breeding in sheep. QPCR was performed to investigate the expression of KISS1 gene in 10 reproductive tissues at different breeds of sheep. Year-round estrous (Small Tail Han sheep, Cele black sheep and Hu sheep) and seasonal estrous (Tan sheep, Sunite sheep and Prairie Tibetan sheep) sheep breeds were selected and Sequenom MassARRAY SNP assay was applied to genotype two loci of KISS1 gene. Then the association was analyzed between KISS1 and litter size in Small Tail Han sheep. The results of gene expression showed that the KISS1 gene was higher in hypothalamus, brain, pituitary and thyroid of Small Tailed Han sheep than that in Prairie Tibetan sheep (P<0.05). From the result of genotyping, both genotype frequency and allele frequency were extremely different between year-round estrous and seasonal estrous sheep (P<0.01) at g.1317523C>T locus. Population genetic analysis showed that the g.1317523C>T locus had moderate polymorphism (0.25<PIC<0.5) in six populations of sheep;The χ^2 test indicated that the g.1317523C>T locus of Small Tail Han sheep, Tan sheep, Sunite sheep and Hu Sheep was under Hardy-Weinberg equilibrium (P>0.05), and the g.1311578G>T locus of Sunite sheep and Hu sheep was under Hardy- Weinberg equilibrium (P>0.05). Association analysis indicated that the polymorphism of two SNPs had no correlation with the litter size in Small Tail Han sheep (P>0.05), while the litter size of TT was higher than GT and GG in the first three births at g.1317523C>T locus. Therefore, KISS1 gene is closely related to the seasonal reproduction of sheep, and the g.1311578G>T locus might provide a basis for litter size trait selection in sheep.
作者
安雪姣
赵生国
潘章源
文禹粱
田志龙
蔡原
储明星
AN Xue-jiao;ZHAO Sheng-guo;PAN Zhang-yuan;WEN Yu-liang;TIAN Zhi-long;CAI Yuan;CHU Ming-xing(Key Laboratory of Animal Genetics,Breeding and Reproduction of Ministry of Agriculture,Instituteof Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China;College of Animal Science and Technology,Gansu Agricultural University,Gansu Lanzhou 730070,China;College of Agriculture and Forestry Science,Linyi University,Shandong Linyi 276005,China)
出处
《中国畜牧杂志》
CAS
北大核心
2019年第6期45-50,共6页
Chinese Journal of Animal Science
基金
国家自然科学基金项目(31472078)
国家肉羊产业技术体系专项(CARS-38)
中国农业科学院科技创新工程(ASTIPIAS13)
