摘要
本研究为初步阐明KiSS-1和RFRP基因对山羊繁殖季节性的作用,选择常年发情济宁青山羊和季节性发情辽宁绒山羊为对比品种,应用RT-PCR和qRT-PCR技术分别研究KiSS-1和RFRP基因组织表达谱以及在下丘脑-垂体-卵巢(子宫)繁殖轴表达差异。结果发现:(1)KiSS-1基因主要表达于山羊下丘脑、垂体、松果体、肾、卵巢、脂肪和甲状腺等组织,RFRP基因主要表达于山羊下丘脑、大脑皮层、小脑、卵巢和垂体等组织,两基因组织表达谱均存在一定的品种特异性;(2)KiSS-1基因在济宁青山羊下丘脑表达量显著高于辽宁绒山羊(P<0.05),而在垂体和卵巢中的表达量两品种间差异不明显(P>0.05);(3)济宁青山羊下丘脑RFRP基因表达显著高于辽宁绒山羊(P<0.05),而济宁青山羊卵巢和垂体中RFRP基因表达明显低于辽宁绒山羊(P<0.05)。研究结果提示,KiSS-1基因可能参与调控山羊季节性繁殖,而RFRP基因是否调控季节性繁殖或以何种方式作用还需要进一步研究。本研究可为山羊KiSS-1和RFRP基因功能研究打下基础,并为揭示山羊繁殖季节性的遗传基础提供参考。
To elucidate the roles of KiSS-1and RFRPgenes in goat,their expression profilings in tissues and the expression levels in hypothalamus-pituitary-ovary(and uterus)were determined between Jining Grey goat(year-round estrus)and Liaoning Cashmere goat(seasonal estrus)by using RT-PCR and qRT-PCR methods,respectively.The results showed that:(i)KiSS-1mRNA was detected in goat hypothalamus,pituitary,pineal,kidney,ovary,subcutaneous fat and thyroid,while RFRP mRNA was detected in goat hypothalamus,cerebral cortex,cerebellum,ovary and pituitary,both of them had different tissue expression profilings between two goat breeds;(ii)KiSS-1mRNA expression level was higher in hypothalamus for Jining Grey than that in Liaoning Cashmere goats(P<0.05),while the expression had no significant difference in ovary and pitui-tary between 2breeds(P>0.05);(iii)The expression level of RFRP mRNA was higher in hypothalamus of Jining Grey than that in Liaoning Cashmere goat(P<0.05),while its expression in ovary and pituitary was opposite between 2breeds(P<0.05).The present study suggests that KiSS-1gene may involve in regulating seasonal breeding in goats,but the effect(or the acting manner)of RFRPgene in goat seasonal breeding need be further researched.The results lay the foundation for elucidating the function of goat KiSS-1and RFRPgenes and provide reference for revealing the genetic mechanism of reproductive seasonality in goat.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2015年第6期924-931,共8页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
中国农业科学院科技创新工程(ASTIP-IAS13)
国家自然科学基金项目(30871773)
国家肉羊产业技术体系专项(CARS-39)
转基因生物新品种培育重大专项课题(2014ZX08010005-003)
