摘要
【目的】探讨甘草酸二钾体外抗MDV的活性及作用机制。【方法】在体外以鸡胚成纤维细胞为MDV增殖模型,利用MTT比色法、噬斑减少法通过抗病毒试验、病毒复制抑制试验、直接灭活试验来评价甘草酸二钾的抗病毒效果;并通过细胞凋亡试验解释其抗病毒机制。【结果】抗病毒试验结果表明,在安全浓度范围内,甘草酸二钾在体外具有较强的抗MDV的活性,其EC50为(893.5±36.99)μg·mL-1,SI>3.36,最大抑制率达94.4%;在病毒复制抑制试验中,甘草酸二钾可以抑制病毒复制的整个过程;病毒直接灭活试验表明甘草酸二钾可直接杀灭病毒粒子且不呈时间依赖性;细胞凋亡试验结果显示甘草酸二钾能够诱导感染MDV的鸡胚成纤维细胞凋亡。【结论】甘草酸二钾在体外具有抗MDV的活性,可以通过诱导感染病毒的鸡胚成纤维细胞凋亡发挥抗病毒作用。
[ Objective ] The aim of the study is to test the antiviml activity and mechanism of dipotassium glycyrrhizinate （DG） against Marek＇s disease virus （MDV） in vitro. [Method] Using MTT test and standard plaque reduction assay on chick embryo fibroblast cells （CEFs）, the antiviral effect of DG against MDV was observed by antiviral assay, time-of-addition assay and virucidal assay, and the antiviral mechanism was tested by cell apoptosis assay. [Result] Antiviral assay showed that DG could reduce the damage of MDV to CEFs, ECs0 was （893.5±36.99）μg·mL^-1, SI〉3.36 and the inhibition ratio was 94.4%, at the maximum with no-cytotoxic concentration. Time-of-addition assay and virucidal assay demonstrated that DG could inhibit the viral replication in the full replication cycle and inactivated MDV particles in non time-dependent manner. Moreover, DG could promote MDV-infected cells apoptosis in vitro. [Conclusion] DG could inhibit MDV on CEFs by promoting MDV-infected cells apoptosis in vitro.
出处
《中国农业科学》
CAS
CSCD
北大核心
2013年第18期3914-3921,共8页
Scientia Agricultura Sinica
基金
山西省科技攻关项目(2010311047
20120311022-1)
关键词
甘草酸二钾
MDV
抗病毒
细胞凋亡
dipotassium glycyrrhizinate
MDV
anti-virus
cell apoptosis
