摘要
目的 观察内毒素（ＬＰＳ）对肝细胞的直接作用及重组人生长激素（ｒｈＧＨ）的干预效果。方法 内毒素诱导肝细胞损害，ＨｅｐＧ２细胞传代培养２４ｈ后，换用含内毒素（２０μｇ／ｍｌ）和／或ｒｈＧＨ、肝细胞生长因子（ＨＧＦ）的新鲜培养液继续培养１６ｈ，观察药物对ＬＰＳ作用的干预效果。结果 ＨｅｐＧ２细胞经ＬＰＳ作用１６ｈ，透射电镜观察既有形态正常的细胞，同时也出现典型的凋亡细胞：表现为胞浆浓缩核凝聚成块状，凋亡小体形成，细胞器（包括线粒体）结构基本正常；ＴＵＮＥＬ标记后胞核呈棕褐色染色，而对照细胞无阳性染色信号，证实ＬＰＳ作用１６ｈ后ＨｅｐＧ２细胞已发生凋亡。将ｒｈＧＨ与 ＬＰＳ同时加入培养液中共同作用１６ｈ，ｒｈＧＨ可明显减少ＨｅｐＧ２细胞经ＬＰＳ诱导的细胞凋亡数量对照组为（９９±０．８）％，ｒｈＨＧ处理组为（３６±５．６）％，Ｐ＜０．００１。结论 重组人生长激素可以抑制ＬＰＳ致肝细胞凋亡作用。
Objective: To investigate the effect of LPS on human hepatocytes and study whether recombinant human growth hormone (rhGH) could protect hepatocytes from apoptosis induced by LPS. Methods HepG2 cells were treated with LPS (20 μg/ml) or LPS and rhGH for 16 hours. The apoptosis of HepG2 cells was detected by terminal deoxynucleotide transferas-mediated dUTP nick end labeling (TUNEL) or electron microscopy. Results HepG2 cells treated with LPS exhibited some specific morphological features of typical apoptosis. the percentage of apoptotic cells in HepG2 cells treated with LPS and hrGH was significantly lower than that treated with LPS (36 ± 5.6)% vs (99 ± 0.8)%, P<0. 001). Conclusions LPS can induce apoptosis of HepG2 cells, and hrGH can downregulate the apoptotic role of LPS on HePG2 cells.
出处
《中华肝脏病杂志》
CAS
CSCD
2001年第2期100-102,共3页
Chinese Journal of Hepatology
关键词
内毒素类
生长激素
细胞凋亡
肝细胞
Lipopolysaccharides
Human growth hormone
Apoptosis
Hepatocytes
