摘要
为了解我国甘肃省、陕西省流行的人副流感病毒3型(Human parainfluenza viruse-3,HPIV-3)的基因特征和流行规律,2009～2011年从上述两省急性呼吸道感染患者中共采集咽拭子标本719份,采用多重反转录聚合酶链反应(Multiplex reverse transcription polymerase chain reaction,多重RT-PCR)方法对常见呼吸道病毒性病原体进行筛查,HPIV-3阳性标本再用巢式PCR(nested polymerase chain reaction,nested PCR)扩增其血凝素-神经氨酶蛋白(Hemagglutinin-neuraminidase,HN)基因,进行序列测定和基因分析。共获得13株HPIV-3HN基因序列,均属于C3亚群,核苷酸差异和氨基酸差异分别为0.2%～2.3%和0～1.1%。与美国、加拿大和澳大利亚的HPIV-3的HN基因序列之间的核苷酸差异和氨基酸差异较大,最大分别为6.0%和3.4%。shaanxi09-2与shaanxi10-H0091、shaanxi10-H0055与gansu11-62110372和shaanxi09-2与BJ/291/09之间存在核苷酸差异分别为0.9%、0.5%和0.6%,但均无氨基酸差异。提示甘肃和陕西两省在不同年份之间存在HPIV-3的持续循环传播。引起2009～2011年甘肃和陕西两省HPIV-3流行的毒株属C3亚群。
To investigate the genetic characterization of Human parainfluenza virus-3 （HPIV-3） circulating in Gansu and Shaanxi Provinces of China, 719 throat swabs were collected from pediatric patients with acute respiratory infections from 2009-2011. Multiplex RT-PCR was used to screen common respiratory vi- ral pathogens. For HPIV-3-positive specimens, nested RT-PCR was used to amplify the HN gene of HPIV-3. The nucleotides of Hemagglutinin-neuraminidase（HN）gene of 13 HPIV-3 positive strains identi- fied in Gansu and Shaanxi Provinces were successfully sequenced and compared with those downloaded from GenBank. The phylogenetic analysis based on the nucleotides sequence of HN gene showed that 13 HPIV-3 strains belonged to sub-cluster C3 with little sequence variation （overall nucleotide divergence of 0.2%-2.3% and amino acid divergence at 0 1.1%）. Compared with the complete gene of HPIV-3 strains from USA, Canada, and Australia, the biggest divergence of the nucleotide and amino acid lovels was 6.0% and 3.4%, respectively. The nucleotide divergence between shaanxi09-2 and shaanxil0-H0091 was 0.9%, while the nucleotide divergence between shaanxil0-H005 and gansu11-62110372 was 0.5%, be- tween shaanxi09-2 and BJ/291/09 was 0.6%. However, there was no amino acid divergence among them. It is likely that HPIV-3 virus had been transmitting in Gansu and Shaanxi Provinces for several years. Hu- man parainfluenza virus-3 （HPIV-3） circulated in Gansu and Shaanxi Provinces from 2009 to 2011 belonged to sub-cluster C3.
出处
《病毒学报》
CAS
CSCD
北大核心
2013年第5期509-514,共6页
Chinese Journal of Virology
基金
自然疫源性传染病病原谱流行规律及变异研究-症候群病毒学研究(项目编号:2012ZX10004201-003)
病毒性传染病病原谱流行规律及变异研究(项目编号:2013ZX10004-202)
重大传染病应急处置检测技术平台(项目编号:2011ZX10004-001)
关键词
人副流感病毒
HN基因
基因特征
序列分析
Human parainfluenza virus
HN gene
Gene characterization
Sequence analysis
