摘要
[目的] 建立霍乱弧菌肠毒素快速检测的检验技术。[方法] 运用巢式聚合酶链反应(NESTED-PCR)检测霍乱弧菌肠毒素基因CTX。[结果] 检测霍乱弧菌特异性强,检测下限达4cfu/mL(100cfu/mL级水平)。[结论] NESTED-PCR检定霍乱弧菌简便、快速、敏感度高且准确性好。
Objective] To establish a rapid technique for detection of pathogenic vibio cholerae. [Method] Amplification of the vibio cholerae's CTX sequence with nested polymerase chain reaction (Nested-PCR). [Result] It's specific and the lowest limit of detection is approximately 4cfu/mL (10 0cfu/mL) by using this molecular biological technique for rapid detecion of cholerae's pathogens. [Conclusion] This molecular biological technique of Nested-PCR is proved to be a rapid,simplified,specific and accurate method for the detection of cholerae's pathogens.
出处
《上海预防医学》
CAS
2000年第1期15-16,共2页
Shanghai Journal of Preventive Medicine
基金
上海市卫生局青年科研基金!( 13 1974Y13 )
