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白细胞介素6在人骨髓瘤细胞系XG-7中的信号转导途径与生物学效应 被引量:3

Signal transduction and biological function of IL-6 in a human myeloma cell line-XG-7
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摘要 目的 研究白细胞介素 6 (IL 6 )依赖型人骨髓瘤细胞系XG 7中IL 6信号转导途径活化与生物学效应之间的关系。方法 分别采用凝胶阻滞电泳和免疫沉淀方法检测参与IL 6信号传递功能的转录因子 (STAT3、NF IL 6 )和蛋白激酶 (JAK1、ERK)在XG 7细胞中的诱导激活状态并确定该细胞中的IL 6信号转导途径 ;进而采用细胞转染技术特异性上调或下调IL 6信号途径的诱导激活状态 ,并观察IL 6在XG 7细胞上生物学效应的改变。结果 IL 6可引发XG 7细胞中Ras/NF IL 6信号转导途径活化 ,但没有出现Jak/STAT途径的诱导激活。Ras途径活化信号的上调与IL 6对XG 7细胞增殖促进作用的增强同步出现 ;而当Ras途径活化被抑制时 ,IL 6对XG 7细胞的生长促进作用明显减弱。结论 Ras/NF IL 6信号转导途径介导了IL 6对XG 7细胞的生长维持和增殖促进作用。 Objective To investigate the relationship between IL 6 signal transduction and its biological function on a human IL 6 dependent myeloma cell line XG 7. Methods Electrophoretic mobility shift (EMSA) and immunopeipitation were used respectively to detect the activation of transcription factors (STAT3 and NF IL 6) and protein kinases (JAK1 and ERK) in the two IL 6 signal transduction pathways by IL 6 in XG 7 cells. Then the cells were transfected with the sense or anti sense expression plasmids of the transcription factors, which could up or down regulate the activation of the signal transduction pathways specially, the changes of the biological function of IL 6 on XG 7 cells was shown by cell number counting and MTT. Results IL 6 can promote the proliferation of XG 7 cells and activate one of the IL 6 signal transduction pathway Ras/NF IL 6; but Jak/STAT signal transduction pathway was not activated at the same conditions. When the activation of Ras/NF IL 6 pathway was up or down regulated, the growth effect of IL 6 on XG 7 cells was strongthened or weakened. Conclusions The proliferation of XG 7 cells in the presence of IL 6 is mediated by the activation of Ras/NF IL 6 signal transduction pathway.
作者 宋伦 沈倍奋
出处 《中华医学杂志》 CAS CSCD 北大核心 2000年第11期845-848,共4页 National Medical Journal of China
关键词 白细胞介素6 多发性骨髓瘤 相对生物学效应 Interleukin 6 Multiple myeloma Relative biological effectiveneL
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