摘要
目的观察头孢他啶阳离子脂质体(cationic liposomal ceftazidime,CLC)复合纳米羟基磷灰石(nano-hydroxyapatite,n-HA)/β-磷酸三钙(β-tricalcium phosphate,β-TCP)对兔慢性骨髓炎的治疗效果。方法 4～6月龄健康新西兰大白兔30只,体重2～3 kg,制备双侧胫骨慢性骨髓炎模型,4周后行大体观察、X线片检查、细菌学及病理组织学检查确定27只造模成功。选取其中24只依据随机对照原则分为4组,每组6只:A组行清创术;B组清创术后给予头孢他啶治疗(90 mg/kg),2次/d,治疗8周;C组清创术后植入游离头孢他啶复合n-HA/β-TCP治疗;D组清创术后植入CLC复合n-HA/β-TCP治疗。治疗前后行X线片检查及Norden评分;治疗后8周处死动物行大体观察,采用Rissing等分类标准行大体病理评分(gross bone pathological score,GBPS);一半标本行组织学观察及Smeltzer评分,另一半行细菌学检查并计算细菌培养阳性率。结果治疗后8周,D组Norden评分明显低于A、B、C组(P<0.05);A、B、C组间比较差异无统计学意义(P>0.05)。治疗后8周大体观察C、D组实验动物感染窦道已愈合,A、B组仍有部分感染窦道残留;GBPS评分C、D组明显低于A、B组(P<0.05),C、D组间差异无统计学意义(P>0.05)。组织学观察示Smeltzer评分C、D组明显低于A、B组(P<0.05);A、B组间及C、D组间差异均无统计学意义(P>0.05)。细菌培养阳性率组C(0)、D组(0)明显低于A组(25.0%)和B组(16.7%),差异有统计学意义(P<0.05)。结论 CLC复合n-HA/β-TCP对于兔慢性骨髓炎具有良好的治疗效果,其治疗效果优于游离头孢他啶复合n-HA/β-TCP。
Objective To observe the effect of cationic liposomal ceftazidime（CLC） combined with nano-hydroxyapatite/β-tricalcium phosphate（n-HA/β-TCP） in the treatment of chronic osteomyelitis of rabbits. Methods Thirty healthy New Zealand white rabbits（4-6 months old;weighing,2-3 kg） were selected to prepare the chronic osteomyelitis models.After 4 weeks,the gross observation,X-ray examination,and bacteriological and histopathological examinations were done;the models were made successfully in 27 rabbits.Of 27 rabbits,24 were randomly divided into 4 groups（n=6）： only debridement was performed in group A;ceftazidime was given（90 mg/kg）,twice a day for 8 weeks after debridement in group B;ceftazidime and n-HA/β-TC were implanted after debridement in group C;and CLC and n-HA/β-TCP were implanted after debridement in group D.Before and after treatments,X-ray examination was done,and Norden score was recorded.At 8 weeks after treatment,the specimens were harvested for gross observation and for gross bone pathological score（GBPS） using Rissing standard;half of the specimens was used for histological observation and Smeltzer scoring,the other half for bacteriological examination and calculation of the positive rate of bacteria culture. Results At 8 weeks after treatment,Norden score of group D was significantly lower than that of groups A,B,and C（P 0.05）,but no significant difference was found among groups A,B,and C（P 0.05）.At 8 weeks after treatment,sinus healed in groups C and D,but sinus was observed in groups A and B;the GBPS scores of groups C and D were significantly lower than those of groups A and B（P 0.05）.The Smeltzer scores of groups C and D were significantly lower than those of groups A and B（P 0.05）.The positive rates of bacteria culture of groups C（0） and D（0） were significantly lower than those of group A（25.0%） and group B（16.7%）（P 0.05）. Conclusion CLC combined with n-HA/β-TCP has good effect in treating chronic osteomyelitis of rabbits,and it ha
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2013年第8期1000-1005,共6页
Chinese Journal of Reparative and Reconstructive Surgery
