摘要
目的:探讨庆大霉素-壳聚糖纳米粒(gentamicin loaded chitosan nanospheres,GS/CS NPs)对兔慢性胫骨骨髓炎的治疗作用。方法:取48只兔制作慢性胫骨骨髓炎模型,造模3周后行大体观察及患肢X线检查。44只兔造模成功后行不同治疗,A组:未特殊治疗;B组:患肢髓腔壳聚糖纳米粒填塞;C组:患肢髓腔庆大霉素粉末填塞;D组:患肢髓腔GS/CS NPs填塞。治疗3周后行患肢X线片检查及Norden评分、髓腔大体观及髓腔内容物细菌学培养,患肢标本行组织学观察及Smeltzer病理评分。结果:治疗3周后A、B、C组均出现明显的慢性骨髓炎X线表现,髓腔脓肿形成,髓腔内容物细菌学培养阳性,组织学观察示中性粒细胞浸润、骨小梁破坏,A、B组尚可见死骨形成,D组治疗后未见明显阳性表现。Norden评分D组较A、B、C组均有统计学意义(P<0.05),Smeltzer病理评分D组较A、B、C组均有统计学意义(P<0.05)。结论:庆大霉素-壳聚糖纳米粒作为局部释药系统治疗兔慢性骨髓炎具有良好的效果。
Objective： To investigate the effect of gentamicin loaded chitosan nanospheres（GS/CS NPs） on the treatment of chronic osteomyelitis of rabbit. Methods： Forty-eight rabbits were used to establish the chronic osteomyelitis model,and observation of affected limb and X-ray were done 3 weeks later. Forty-four rabbits had symptoms and signs of chronic osteomyelitis and were divided into 4 groups. Group A has no treatment, group B was treated with chitosan nanospheres, group C was treated with gentamicin sulfate, and group D was treated with GS/CS NPs. The X-Ray,observation of the bone marrow cavity,bacterial culture and the HE staining were done after 3 weeks of treatment. Results： After 3 weeks of treatment,the X-Ray of groups A-C showed apparent signs of chronic osteomyelitis,the formation of abscess were observed in bone marrow cavity, and the cultures of the Staphylococcus aureus were positive. The HE staining of groups A-C showed neutrophil accumulation, and the formation of dead bone were observed in groups A-B. The Norden and Smehzer scores showed significant differences between group D and groups A-C （both P 〈 0.05）. Conclusion： GS/ CS NPs has significant effects on the treatment of rabbit chronic osteomyelitis as local drug delivery system.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第2期208-211,共4页
Journal of Nanjing Medical University(Natural Sciences)
关键词
庆大霉素
壳聚糖
纳米粒
兔
骨髓炎
gentamicins
chitosan
nanospheres
rabbit
osteomyelitis
