摘要
背景:显微CT(Micro-CT)是一种非破坏性的3D成像技术,可以在不破坏样本的情况下清楚地了解样本的内部显微结构,其分辨率极高,空间分辨率达到1-10μm,部分先进机型甚至达0.25μm,无论是数据分析还是图像处理,Micro-CT目前都已在骨组织工程研究中被广泛应用。目的:在兔胫骨骨髓炎建模过程中,以Micro-CT作为辅助观察工具,分析兔胫骨因炎症产生的结构变化以验证骨髓炎模型是否建立成功。方法:取新西兰大白兔20只,其中5只作为对照组,其余15只建立右侧胫骨骨髓炎模型,分别在2,4,6周进行影像学表征(n=5)。术后观察兔的进食、作息及伤口愈合情况;采用X射线活体成像检测兔胫骨骨髓炎模型形成过程中的骨组织变化;使用Micro-CT对建模后患肢进行逐层扫描,获得相应断层图像并对其进行三维重建,计算各组样本的骨体积、骨密度等参数。结果与结论:①大体观察:第4周起可见造模处周围有脓性液体及组织,并出现炎性反应;第6周可见大面积骨质增生;②X射线片观察:随时间推移可见模型组的肿胀程度和骨膜反应愈发严重,死骨形成变多;③Micro-CT分析:断层图像可见,前4周骨质逐渐出现疏松和增生现象;6周后建模成功的兔骨皮质明显被破坏且严重增生,炎症蔓延至骨髓腔;三维重建后可见6周骨髓炎建模兔骨窗周围有大量死骨形成且骨膜反应剧烈,整体明显肿胀,骨质增生严重;内部松质骨结构严重破坏变形,骨小梁大面积破损缺失,且炎症已蔓延至整根胫骨,骨内膜及骨关节面有显著损伤,皮质骨增生且出现空隙;对炎症与正常骨参数进行计算发现,炎症后骨体积均有所上升,皮质骨密度下降,松质骨密度上升;④结果表明,Micro-CT可行断面成像、三维重建及骨参数定量分析,其优势明显弥补了X射线片与大体观察的不足,为骨髓炎相关骨组织工程研究提供了实验依据及新思路。
BACKGROUND:Micro-computed tomography(micro-CT)is a non-destructive three-dimensional imaging technology,which can clearly observe the internal microstructure of a sample without damaging.Its resolution is very high,and the spatial resolution reaches 1-10μm,even 0.25μm in some advanced models.Micro-CT has been widely used for data analysis and image processing in bone tissue engineering research.OBJECTIVE:To analyze the inflammation-induced structural changes of the rabbit tibia using micro-CT to verify whether a rabbit model of osteomyelitis is successfully established.METHODS:Twenty New Zealand white rabbits were selected,five of which were taken as the control group,and the remaining fifteen rabbits were used to establish the right tibia osteomyelitis model.After modeling,imaging characterization was conducted in the rabbit models(n=5 at each time point)at 2,4,and 6 weeks.Feeding,pattern of sleeping,and wound healing in rabbits were observed postoperatively.X-ray in vivo imaging was used to detect the changes of bone tissue during the modeling of rabbit tibial osteomyelitis.Micro-CT was used to scan the affected limb layer by layer to obtain the corresponding crosssectional images,followed by three-dimensional reconstruction.Bone volume and bone mineral density were calculated in each group.RESULTS AND CONCLUSION:Gross observation:Purulent liquids and tissues could be found around the modeling site from the 4th week,and inflammatory reactions occurred.Large-area hyperostosis was observed at the 6th week.X-ray imaging observation:the swelling degree and periosteal reaction in the model group became gradually serious over time,and dead bones proliferated a lot.Micro-CT analysis:The cross-sectional images showed that osteoporosis and hyperplasia gradually appeared during the first 4 weeks.After 6 weeks of modeling,the rabbit cortical bone was significantly damaged and seriously proliferated,and inflammation spread to the medullary cavity.Through the three-dimensional reconstructions,a mass of dead bones formed a
作者
李雨帆
林明玥
王晨鑫
张瑞
李玉宝
陈立
邹琴
Li Yufan;Lin Mingyue;Wang Chenxin;Zhang Rui;Li Yubao;Chen Li;Zou Qin(Analysis and Testing Center,Sichuan University,Chengdu 610064,Sichuan Province,China;Nano-biomaterials Research Center,Sichuan University,Chengdu 610064,Sichuan Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2022年第18期2874-2880,共7页
Chinese Journal of Tissue Engineering Research
基金
四川省重大科技专项(2020ZDZX0008),项目负责人:邹琴
成都市科技项目(2019-YF05-00020-SN),项目负责人:邹琴。
关键词
显微CT
胫骨骨髓炎
骨微观结构
三维重建
定量分析
兔
micro-computed tomography
tibial osteomyelitis
bone microstructure
three-dimensional reconstruction
quantitative analysis
rabbit
