摘要
目的观察脾虚状态下大鼠有机阴离子转运肽oatp2b1基因和蛋白表达,探讨oatp2b1在湿浊转运中的作用。方法 24只大鼠随机分为正常组、高脂组、劳倦组,每组8只。造模成功后,每只大鼠取肺、脾、肝、肾、胃、小肠、大肠组织各1块。劳倦组单号日期采用特制束缚筒,每次3h,双号日期令大鼠在冷水(10±1)℃中游泳7min,连续12周。模型组、正常组常规标准大鼠颗粒饲料喂养12周,高脂组大鼠以高脂饲料喂养12周,均自由饮食饮水。采用RT-PCR、Western Blot法检测肺、脾、肝、肾、胃、小肠、大肠组织oatp2b1mRNA、蛋白表达。结果高脂组肾、肝oatp2b1 mRNA含量高于正常组及劳倦组(P<0.01,P<0.05);正常组oatp2b1mRNA表达量由高至低为肝>肺>脾>大肠>小肠>肾>胃;劳倦组oatp2b1 mRNA表达量由高至低为肝>肺>大肠>脾>肾>胃>小肠;高脂组oatp2b1 mRNA表达量由高至低为肝>肺>脾>小肠>肾>大肠>胃。肺组织oatp2b1蛋白表达量由高至低为劳倦组>正常组>高脂组(P>0.05)。脾组织oatp2b1蛋白表达由高至低为高脂组>正常组>劳倦组(P>0.05);肾组织oatp2b1蛋白表达量由高至低为正常组>劳倦组>高脂组(P>0.05);肝组织oatp2b1蛋白表达量由高至低为正常组>高脂组>劳倦组(P>0.05);其中胃、大肠、小肠中oatp2b1蛋白表达量极低。正常组oatp2b1蛋白表达由高至低为肺>脾>肝,肾>胃、大肠、小肠;劳倦组oatp2b1蛋白表达由高至低为:肺>脾>肾>肝>胃、大肠、小肠;高脂组oatp2b1蛋白表达由高至低为:脾>肺>肾>肝>胃,大肠,小肠,差异无统计学意义(P>0.05)。结论肝、肾等脏器在脾失健运状态下的湿浊转运中可能发挥重要作用,oatp2b1是机体参与湿浊运化的物质基础之一。脾主运化湿浊的功能,可能不仅包括胃肠道的功能在内,而且也可能包括一部分肝脏及肾脏的功能。
Objective To explore roles of mRNA and protein expressions of organic anion transporting polypeptide （oatp2bl） of rats with high fat diet and overstrain induced Pi deficiency syndrome in the transporting of damp turbidity. Methods Totally 24 SD rats were randomly divided into three groups, i.e., the normal group, the overstrain group, and the high fat diet group, 8 in each group. After successful modeling, one piece of tissues such as spleen, kidney, liver, lung, stomach, small intestine, and large intestine was taken from each rat. Rats of the overstrain group were bonded by specially made bondage cylinder, 3 h each time on odd days, and forced to swim in cold water （10 ~1 ） ~C for 7 min on even days alternatively for twelve weeks. Rats in the model group and the normal group were fed with standard rou- tine granular forage for 12 weeks. Rats in the high fat diet group were fed with high fat forage for twelve weeks. All rats drank and ate freely. The mRNA and protein expressions of oatp2bl were detected in the seven tissues using RT-PCR and Western blot. Results The mRNA expression of oatp2bl in liver and kidney tissues of rats in the high fat diet group was higher when compared with that of the normal group and the overstrain group （P 〈0.01, P 〈0.05）. The oatp2bl mRNA expression in the normal group was sequenced from high to low as liver 〉lung 〉spleen 〉larger intestine 〉small intestine 〉 kidney 〉stomach. The oatp2bl mRNA expression in the overstrain group was sequenced from high to low as liver 〉lung 〉 larger intestine 〉spleen 〉 kidney 〉 stomach 〉small intestine. The oatp2bl mRNA expression in the high fat diet group was sequenced from high to low as liver 〉 lung 〉 spleen 〉 small intestine 〉 kidney 〉 larger in- testine 〉stomach. The oatp2bl protein expression in the lung tissue was sequenced from high to low as the overstrain group 〉the normal group 〉the high fat diet group （P 〉0.05）. The oatp2bl protein expres- sion in the spleen tissue was sequence
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2013年第7期953-957,共5页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助项目(No.81072806)
国家"十一五"科技重大专项(No.2009ZX10005-016)
广东省自然科学基金资助项目(No.9151008004000030)
关键词
湿浊
伤脾
有机阴离子转运肽2b1
damp
impairment of Pi
organic anion transporting polypeptide 2bl
