摘要
目的检测吸毒者细胞免疫、体液免疫、非特异度免疫的各项指标,从而研究其整体免疫功能的改变以及与吸食毒品种类的相关性。方法取47例吸毒者(其中26例海洛因成瘾者、21例冰毒成瘾者)以及23例健康人外周血,采用流式细胞术(FCM)检测其T细胞亚群(CD3+、CD4+、CD8+)、B细胞以及NK细胞百分率;采用ELISA法测IL-2血清含量;采用免疫浊度法检测其血清中抗体IgG、IgA、IgM以及补体C3、C4。结果吸毒者CD3+T细胞、CD4+T细胞、B细胞、IgM及C3较健康人均有显著性增高(P<0.01或P<0.05);吸毒者NK细胞及IL-2较健康人均有显著性降低(P<0.01);海洛因组与冰毒组较健康对照组免疫功能均有显著变化,但两者之间比较差异无统计学意义(P>0.05)。结论吸毒可引起机体免疫系统广泛而严重的紊乱或损伤,吸毒者细胞、体液及非特异度免疫均有明显改变,毒品种类对其免疫功能改变的影响则不大。
Objective To detect the indicators of cellular immunity,humoral immunity,non-specific immune in drug addicts,so as to study the overall changes in immune function,and the correlation with the types of drugs.Methods The percentages of T lymphocyte subsets（CD3＋,CD4＋,CD8＋）,B cells and NK cells were detected with flow cytometry（FCM） in 47 cases of drug addicts（including 26 cases of heroin addicts,21 cases of methamphetamine addicts） and 23 normal peasons,the levels of IL-2 in serum were detected by ELISA,the levels of IgG,IgA,IgM,C3,C4 in serum were detected by immune turbidimetric method.Results The percentages of CD3＋T cells,CD4＋T cells,B cells and the levels of IgM and C3 in drug addicts were significantly increased compared with normal control group（P＆lt;0.01 or P＆lt;0.05）.The percentages of NK cells and the levels of IL-2 were significantly decreased compared with normal control group（P＆lt;0.01）.Immune function of Heroin group and methamphetamine group were significantly changed compared with normal control group,but there was no statistically significant difference between the two（P＆gt;0.05）.Conclusion Drugs can cause serious disorder or injury in immune system.The cellular immunity,the humoral immunity and the non-specific immune in drug addicts are changed significantly.The types of drugs have no effect on immune function of drug addicts.
出处
《国际检验医学杂志》
CAS
2013年第10期1227-1229,共3页
International Journal of Laboratory Medicine
基金
上海交通大学医学院附属精神卫生中心院级课题(2010-YJ-07)
关键词
吸毒
免疫功能
流式细胞术
淋巴细胞亚群
drug abuse
immune function
flow cytometry
lymphocyle subsets
