摘要
目的调查2012年河南省丙型肝炎病毒（HCV）感染状况和基因型分布。方法采用多阶段随机抽样的方法，于2012年3—6月在河南省抽取1～74岁常住人群32203名，对其进行问卷调查，收集一般人口学信息、既往病史以及危险因素的暴露史等。采集5ml静脉血，经ELISA和巢式RT—PCR法分别进行HCV抗体和HCVRNA检测；并对HCVRNA阳性样本进行非结构蛋白5区（NS5B）基因扩增和测序，对所测序列进行系统进化树分析和基因分型；分析不同特征调查对象抗-HCV、HCV—RNA阳性率及HCV基因亚型分布的差异。结果在32203名调查对象中，抗-HCV和HCVRNA阳性率分别为0．48％（153／32203）和0．24％（78／32203），其中男性分别为0．42％（65／15634）和0．23％（36／15634），女性分别为0．53％（88／16569）和0．25％（42／16596），性别间阳性率差异均无统计学意义（χ2值分别为2．26和0．18，P值均〉0．05）。NS5B基因分型与分子进化分析结果显示，在71份HCVRNA阳性样本中，共有6种亚型，分别为1b、6a、3a、2a、3b和1a，所占比例分别为56．3％（40／71）、19．7％（14／71）、11．3％（8／71）、8．5％（6／71）、2．8％（2／71）和1．4％（1／71）；感染者HCV基因亚型主要集中在1b和6a这两个分支上，两种亚型的Bootstrap值均为0．95。结论2012年河南地区HCV流行处于较高水平，基因型亚型以1b和6a型为主。
Objective To investigate the prevalence and distribution of hepatitis C virus （HCV） genotypes in Henan province in 2012. Methods A total of 32 203 permanent residents （ 1 to 74 years old） in Henan were recruited using multi-stage random samping method from March to June 2012. All participants were asked to complete a questionnaire to collect demographic information, past medical history and the exposure history of risk factors. A blood sample of 5 ml was collected at the same time. The condition of anti-HCV and HCV RNA was determined through the ELISA test and nested RT-PCR. HCV RNA positive samples were further subject to the nonstmctural protein 5 region （NSSB） gene amplification and sequencing. The sequence was amplified for the phylogenetic tree and genetic analysis. The differences of the positive rate of anti-HCV and HCV RNA and the HCV genetic subtype distribution in different respondents＇ characteristics were analyzed. Results Among 32 203 subjects, the overall positive rate of anti-HCV and HCV RNA were 0. 48% （ 153/32 203） and 0. 24% （78/32 203） ,in which men were 0.42% （65/15 634）, and0.23% （36/15 634）,and women were 0.53% （88/16 569） and 0.25% （42/16 596）.The differences between men and women were not statistically significant （X2 values were 2. 26, 0. 18, respectively, both P values 〉 0. 05 ）. The results of NSSB genotyping and molecular evolution analysis showed that there were six subtypes in the 71 HCV RNA positive samples. In those six subtypes, the proportion of genotypes lb,6a,3a,2a,3b and la were 56. 3% （40/71）, 19. 7% （ 14/71 ）, 11.3% （8/71） ,8. 5% （6/71）, 2. 8% （2/71） and 1.4% （ 1/71 ）, respectively. The HCV genetic subtypes of infestor were mainly present with two branches of lb and 6a,and the two subtypes Bootstrap values were 0. 95. Conclusion The prevalence of HCV infection was high in Henan. The major HCV genotypes in patients with HCV infection were lb and 6a.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2013年第6期518-522,共5页
Chinese Journal of Preventive Medicine
基金
2012年河南省卫生厅“四病”调查项目
河南省创新型科技人才队伍建设工程
