摘要
目的:观察上海地区慢性丙型肝炎(慢丙肝)患者的丙肝病毒(HCV)基因型分布情况。方法:提取血清HCV RNA,套式PCR扩增方法扩增目的片段,纯化后直接测序。结果:71例血清标本检测示,1b型占87.32%(62/71),2 a型占12.68%(9/71),共有22个突变。1b型的突变率为33.87%(21/62),2 a型的突变率为11.11%(1/9)。两种基因型突变率比较,具统计学差异(P<0.05)。结论:该研究中HCV基因型为1b和2 a两型,1b型占多数,1b型的突变率高于2 a型。
Objective： To study the distribution of HCV Genetype in Shanghai. Methods： HCV RNA was extracted and amplified by nested PCR, then purified and sequenced directly. Results： 1b genetyep aeounted for 87. 32% （62/71）, 2a genetype for 12.68% （9/71） . There were 22 mutation in all samples 33.87% in lb genetype and 11.11% in 2a genetype. Conclusion： The study shows that there are lb and 2a genetypes, and lb genetype is in the majority. The mutation rate of 1 b genetype is more than 2a genetype （ P 〈 0. 05 ） .
出处
《中西医结合肝病杂志》
CAS
2006年第6期360-361,365,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
关键词
肝炎病毒
丙型
基因型分布
流行病学分析
hepatitis, virus C （HCV）
genetype distribution
Shanghai district
