摘要
目的 探索一种新的不受血清抗体回忆反应影响的血清学幽门螺杆菌 (Hp)检测方法。方法 采用生物素 亲和素酶联免疫法测定血清Hp可溶性抗原 ,判断患者Hp感染状况 ,并与快速尿素酶试验、组织切片染色、血清Hp抗体 3种检测方法进行比较 ,对该方法的可靠性进行评估。结果血清Hp可溶性抗原 (HpSAg)检测诊断敏感性为 93 0 2 % ,特异性为 87 88% ,阳性预测值为 90 91% ,阴性预测值为 90 6 3%。10 3例慢性胃炎、消化性溃疡及其他消化道疾病接受胃镜检查患者Hp检测结果显示 ,血清HpSAg检测阳性率与非血清学Hp检测方法结果基本一致 (P >0 0 5 )。结论 HpSAg检测作为一种新的血清学Hp检测方法 ,具有检测快速、经济、准确、便于大规模普查等优点 ,且不受抗体回忆反应影响 ,结果稳定、特异性高 ,可用于Hp的临床诊断、根除疗效观察和随访复查等 ,也适合于儿童及孕妇检测。
Objective To explore a new serological method for detecting Helicobacter pylori(Hp) infection which cannot be affected by memorizing reaction of serum Hp antibody after eradication. Methods Serum soluble antigen of Hp was detected by using avidin biotin ELISA technique to evaluate the status of Hp infection and to compare with rapid urease test(RUT), histologic examination and serology. Results The sensitivity, specificity, positive predictive value and negative predictive value were 93.02%, 87.88%, 90.91% and 90.63% respectively. The prevalence rate of serum Hp soluble antigen in 103 patients undergoing endoscopy was similar either with serological or non serological methods (P>0.05). Conclusion The detection of serum Hp soluble antigen could be used as a new serological method which is accurate, convenient, not being affected by memorizing reaction of serum antibody, more sensitive, more specific and suitable for clinical diagnosis, evaluation of eradication and follow up of Hp as well as for detection in children and pregnant women.
出处
《中华内科杂志》
CAS
CSCD
北大核心
2000年第8期521-523,共3页
Chinese Journal of Internal Medicine
