摘要
背景:寻找操作简便的非侵入性检测方法对于幽门螺杆菌(H.pylori)现症感染的诊断非常重要。目的:评估应用胶体金免疫层析技术的商用血清学试剂盒在诊断H.pylori现症感染中的应用价值。方法:167名健康志愿者纳入本研究。采集血标本,以Assure^(?)H.pylori快速检测试剂盒行免疫层析。同时行^(13)C-尿素呼气试验(^(13)C-UBT)和内镜活检组织病理学检查,两项检查均阳性定义为H.pylori感染,两项检查均阴性定义为H.pylori阴性。结果:根据诊断标准,147例纳入统计分析者的H.pylori感染率为65.3%,Assure^(?)H.pylori快速检测的现症感染阳性率为68.7%,其诊断敏感性、特异性、阳性预测值、阴性预测值和准确性分别为96.9%、84.3%、92.1%、93.5%和92.5%。结论:Assure^(?) H.pylori快速检测是一种简单、快速、可靠的非侵入性H.pylori现症感染诊断方法,在流行病学调查中有很好的应用前景。
Background： To search a noninvasive and less cumbersome method for the diagnosis of current Helieobaeter pylori （H. pylori） infection is very important. Aims： To appraise the value of a commercial serological kit using colloidal gold immunoehromatographie method for the diagnosis of current H. pylori infection. Methods： One hundred and sixty-seven healthy volunteers were recruited in this study. All of them had venous blood drawn for H. pylori serology test by using Assure H. pylori Rapid Test, and each of them underwent ^13C-urea breath test （^13C-UBT） and endoscopy with biopsy and histopathology. Positive results of both ^13C-UBT and histopathology were defined as having H. pylori infection, and negative results of the two examinations were defined as no infection. Results： Among the 147 volunteers recruited in statistical analysis, 96 eases （65.3%） were diagnosed as having H. pylori infection by ^13C-UBT combined with histopathology, whereas the positivity rate of current H. pylori infection of the commercial serological kit was 68.7%. The sensitivity, specificity, positive and negative predictive value, and accuracy of Assure H. pylori Rapid Test were 96.9%, 84.3%, 92.1%, 93.5% and 92.5%, respectively. Conclusions： Assure H. pylori Rapid Test is an optimal test for the diagnosis of current H. pylori infection with the advantages of simple, rapid, reliable and noninvasive. It may be useful in the epidemiologieal studies of current 14. pylori infection.
出处
《胃肠病学》
2007年第9期551-553,共3页
Chinese Journal of Gastroenterology
基金
江苏省"135工程"重点学科基金资助(项目编号:135-04)
关键词
螺杆菌
幽门
诊断
血清学
尿素呼气试验
组织学
Helicobacter pylori
Diagnosis, Serology
Urea Breath Test
Histology
