摘要
以鹿蹄草为原料,利用微波双水相辅助提取技术进行提取,在单因素实验的基础上对提取条件进行了考察,根据BBD(Box-Behnken Design)实验设计原理,采用3因素3水平的响应面分析法,以鹿蹄草中金丝桃苷(hy-perin)为指标,对提取过程进行优化,得到最佳工艺参数为:提取温度50℃,液固比36∶1,(NH4)2SO4浓度22%,提取时间22 min,微波功率300 W,提取次数3次。在最佳提取条件下,金丝桃苷的提取率可达0.982 mg.g-1,纯度可以达0.443%。
Based on the preliminary experiments, a three-var/able, three-level Box-Behnken design was performed to study the aqueous two-phase system （ATPS） extraction of hyperin from Pyrola calliantha H. Andr.. The optimum parameters were as follows： extraction temperature 50℃, liquid /solid ratio 36：1, （NH4） 2SO4 concentration 22%, extraction time 22 Inin, extraction power 300 W and three extraction cycles. Under the optimal conditions, the average extraction yield of hyperin was 0. 982 mg· g^-1 and the purity was 0. 443%. This study is significant for the application of technology and utilization of P. calliantha H. Andr. an aqueous two-phase system （ATPS） extraction
出处
《植物研究》
CAS
CSCD
北大核心
2013年第3期367-370,共4页
Bulletin of Botanical Research
基金
东北林业大学学术名师支持计划(PFT-1213-14)
