摘要
采用超声波辅助乙醇/盐双水相萃取技术对栀子中京尼平苷的提取分离效果进行了系统研究,在单因素试验基础上,采用响应曲面试验设计进行优化,进一步比较了该方法与超声波溶剂提取法、热回流提取法等传统方法对京尼平苷的提取效果。结果表明:超声波辅助乙醇/盐双水相萃取的最佳工艺条件为构建10.0 g乙醇/NaH_2PO_4双水相体系,其中加入无水乙醇4 m L(即体系中乙醇质量分数为31.56%),NaH_2PO_4质量分数为15.00%,超声波温度为30℃,超声波时间为30 min,栀子干粉加入量为0.03 g,上相中京尼平苷的得率为51.77 mg/g。该方法提取时间短、提取温度较低,而且得率较高,对栀子中京尼平苷的提取效果明显优于超声波溶剂提取法和热回流提取法等传统方法。
An ultrasonic-assisted technology combined with ethanol/NaH2 PO4 aqueous two-phase system （UAATPE） was developed for the extraction and purification of geniposide （GP） from gardenia fruits. Based on the results of single factor experiments, the Box-Behnken Design combined with response surface methodology was applied to optimize the operating parameters. Furthermore, the comparisons with ultrasonic solvent extraction, heat reflux extraction, and ultrasonic-assisted aqueous two-phase extraction were also assessed. GP was mainly extracted into the top ethanol-rich phase. And the optimal experimental conditions were 10.0 g ethanol/sah aqueous two-phase system, NaH2PO4 mass fraction 15.00%, ethanol 4 mL （ethanol mass fraction 31.56% ）, extraction temperature 30 ℃, extraction time 30 min and sample loading 0. 03 g. And the maximum yield of GP in top phase was 51.77 mg/g. The comparison results indicated that ultrasonic-assisted aqueous two-phase extraction was more efficient and environmentally friendly than ultrasonic solvent extraction and heat reflux extraction, for the technique had the shorter extraction time, the lower extraction temperature and the higher extraction efficiency.
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2017年第2期93-100,共8页
Chemistry and Industry of Forest Products
基金
国家自然科学基金资助项目(31560105)
湖南省自然科学基金资助项目(14JJ6037)
关键词
双水相体系
超声波辅助
栀子
京尼平苷
aqueous two-phase systems
ultrasonic assisted
gardenia fruits
geniposide
