摘要
目的:研究超声波辅助兖州卷柏生物碱提取工艺,以及兖州卷柏生物碱对小鼠H22肝癌细胞的增殖抑制作用。方法:采用超声辅助乙醇浸提法对兖州卷柏生物碱进行提取,并设计正交实验对提取工艺进行优化。采用MTT法研究兖州卷柏生物碱对小鼠H22肝癌细胞增殖抑制活性。结果:超声辅助兖州卷柏提取最佳工艺为:乙醇浓度70%、固液比1:40、超声时间60min、超声功率400W和超声温度60℃。最高提取率达1.18%。兖州卷柏生物碱对小鼠H22肝癌细胞增殖抑制效果随浓度增大和培养时间增长而增加,其中细胞培养48h,生物碱浓度为8mg/mL时,对肝癌细胞增殖抑制率达53.25%。结论:超声辅助法是兖州卷柏生物碱提取的有效方法,兖州卷柏生物碱对肝癌H22细胞有明显抑制作用。
Objective：An ultrasonic technology was employed to extract alkaloid from Selaginella involvens（ASl）. Furthermore,anti-proliferation activity on hepatocellular carcinoma cell H22 of mouse in vitro of ASI was observed. Methods=The alkaloids from Selaginella involvens were extracted by using ultrasonic-assisted ethanol. An orthogonal experiment was carried out to optimize the extraction technology. Furthermore,the proliferation inhibiting of hepatocellular carcinoma cell H22 was determined by MTT assay. Results=The optimum extraction conditions of polysaccharides was as follows.ethanol concentrations of 70% ,solid-liquid ratio of 1：40,ultrasonic duration of 60min,ultrasonic power of 400W and ultrasonic temperature of 60~C. The highest alkaloid extraction rate reached 1.18%. H22 cell proliferation rate gradually increased with the alkaloid concentration and time increasing. Under the condition of alkaloid condensation of 8mg/mL,the growth inhibition was the highest after 48h and reached 53.25%. Conclusions.Ultrasonic method was a very effective method of extraction alkaloid from Selaginella involvens. The alkaloid could inhibit the growth of hepatocellular carcinoma cell H22 of mouse in vitro.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第10期265-268,共4页
Science and Technology of Food Industry
基金
泉州市科技重点项目(2012Z76)
关键词
兖州卷柏
生物碱
肝癌
抗肿瘤
Selaginella involvens alkaloid
hepatoma anti-proliferation
