摘要
通过单因素和响应面分析试验设计,以总皂苷得率为响应值,对四齿四棱草总皂苷超声提取工艺进行优化。同时采用紫外分光光度法检测四齿四棱草总皂苷对DPPH自由基、ABTS自由基的清除能力和总的还原能力。结果表明:优化工艺条件为提取时间40 min、液料比301(m L/g)、乙醇浓度77%,该条件下四齿四棱草总皂苷得率为3.1 mg/g。四齿四棱草总皂苷对DPPH自由基的清除作用较强,总的还原能力及对ABTS自由基的清除作用相对较弱。
To investigate ultrasonic method of total saponins from S. tetradonta and study the antioxidant activities. By using the single factor experiment and response surface methodology,taking he total saponin yields as the response value,the extraction procees was optimized. Meanwhile,UV spectrophotometry were used to determine the abilities of total saponins from S. tetradonta on scavenging DPPH·,ABTS· and FRAP assay. The results indicated that the optimal extraction conditions were as follows： ultrasonic time 40 min,ratio of water to material 30 m L / g,and ethanol concentration 77%. Under these conditions,the content of total saponins reached 3. 1 mg / g. The total saponins from S. tetradonta had a strong ability to scavenge of DPPH ·. And its total reducing power assays and ABTS· scavenging effect were relatively weak.
出处
《食品与机械》
CSCD
北大核心
2016年第7期141-145,共5页
Food and Machinery
基金
国家自然科学基金项目(编号:81373668)
重庆市卫生局科技项目(编号:2012-2-144)
关键词
四齿四棱草
总皂苷
抗氧化性
紫外分光光度法
S.tetradonta
total saponins
antioxidant activity
UV spectrophotometry
