摘要
目的:建立人参茎叶皂苷胶囊中人参皂苷Rg1和人参皂苷Re的含量测定方法。方法:采用HPLC-ELSD法,Lichro-spher NH2分析柱(250 mm×4.6 mm,5μm),流动相为乙腈-水(80∶20);蒸发光散射检测器漂移管温度为90℃,氮气为载气,流速为2.1 L.min-1。结果:人参皂苷Rg1在0.203～4.060μg,人参皂苷Re在0.212～4.240μg,进样质量的对数值与峰面积的对数值呈良好线性关系;制剂中两种成分的平均回收率分别为101.2%(RSD=2.5%)和100.7%(RSD=2.4%)。结论:该方法简便、准确,分离效果好,无干扰,可作为人参茎叶皂苷胶囊质量控制依据。
Objective： To determine ginsenoside RgI and ginsenoside Re in total ginsenoside in stem and leaf capsules by HPLC-ELSD. Method： A Liehrospher NH2 column （2250 mm×4.6 mm,5μm）was used. The mobile phase consisted of acetonitrile-water （ 80： 20）. The temperature of the drift tube was 90℃ and the flow rate of nitrogen was 2.1 L·min^-1. Result： The linear range of the two components was 0. 203-4.060 μg and 0. 212-4. 240 μg, respectively. The average recovery was 101.2% （ RSD = 2.5% ） and 100.7% （RSD = 2.4% ） , respectively. Conclusion： The method is reliable and precise with promising separation, and can be used as the quantitative analysis method for total ginsenoside in stem and leaf capsules.
出处
《中国药师》
CAS
2013年第4期546-548,共3页
China Pharmacist
