摘要
目的建立分光光度法测定宁神颗粒中总皂苷含量。方法采用Amberlite-XAD-2大孔树脂层析柱富集法纯化总皂苷,以香草醛-高氯酸溶液为显色剂使显色,采用分光光度法(λ=560nm)对宁神颗粒中总皂苷的含量进行测定。结果宁神颗粒中总皂苷浓度在31.76～190.6μg/mL范围内呈良好线性关系(r=0.9996);低、中、高3个浓度的平均回收率分别为97.7%、98.9%和99.2%,相对标准偏差(relative standard deviation, RSD)小于2.2%。本方法的检出限为3.4μg/g,定量限为11.2μg/g。结论本方法简便、准确、成本低,可用于宁神颗粒中总皂苷的含量测定,对产品进行质量控制。
Objective To establish a method for the determination of total saponins in the Ningshen granules by spectrophotometry. Methods Total saponins was purified by Amberlite-XAD-2 macroporous resin column enrichment method, color development with vanillin-perchloric acid solution as color developer. The content of total saponins in Ningshen granules was determined by spectrophotometry（λ=560 nm）. Results The linear range of total saponins in Ningshen granules was 31.76-190.6 μg/mL（r=0.9996）. The average recoveries of low, medium and high concentrations were 97.7%, 98.9% and 99.2%, respectively. The relative standard deviation（RSD） was less than 2.2%. The limit of detection was 3.4 μg/g and the limit of quantitation was 11.2 μg/g. Conclusion This method is simple, accurate and has low cost, which can be used for determining the content of total saponins in Ningshen granules and controlling the quality of products.
作者
董宇
姜鑫
刘静
王震红
DONG Yu;JIANG Xin;LIU Jing;WANG Zhen-Hong(Liaoning Institute for Drug Control,Shenyang 110036,China)
出处
《食品安全质量检测学报》
CAS
2018年第19期5208-5211,共4页
Journal of Food Safety and Quality
关键词
总皂苷
宁神颗粒
分光光度法
total saponins
Ningshen granules
spectrophotometry
