摘要
目的:用 HPLC—ELSD 测定三七药材中三七皂苷 R_1及人参皂苷 Rg_1、Re、Rb_1的含量。方法:色谱柱为 Lichrospher NH_2柱(4.6 mm×250 mm,5μm),流动相为乙腈-水(80:20);漂移管温度为90℃,载气流速为2.1 L·min^(-1)。结果:三七皂苷 R_1及人参皂苷 Rg_1、Re、Rb_1分别在0.316～1.58μg、1.21～6.05μg、0.448～2.24μg及1.49～7.47μg呈良好线性关系;药材中4种成分的平均回收率(n=6)分别为102.2%(RSD=2.6%)、99.4%(RSD=2.2%)、101.8%(RSD=2.5%)及97.6%(RSD=2.4%)。结论:该方法简便、准确,分离效果好,无干扰,可用于三七药材的质量评价。
Objective：To establish the quantitative method of notoginsenoside R F,ginsenoside Rg1,Re and Rb1 in Radix Notoginseng by HPLC - ELSD. Method ： The column used was a Lichrospher NH2（4.6mm×250mm,5μm） packed with a 5μm stationary phase. The mobile phase consisted of acetonitrile -water（80： 20）. The temperature of drift tube was 90℃ and the nebulizer nitrogen flow rate was 2.1L·min^-1. Result： The linear ranges of the three components were 0.316 - 1.58μg,1.21 -6.05μg,0.448 -2.24μg and 1.49 -7.47μg respectively. The average recoveries of the four components（ n = 6） were 102.2% （ RSD = 2. 6% ） ,99.4% （ RSD = 2.2% ）, 101.8% （ RSD = 2.5% ） and 97.6% （ RSD = 2.4% ） respectively. Conclusion： It is proved that the method with HPLC - ELSD is reliable,simple, well separated and precise,that can be used as principles of quantitative analysis.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2008年第3期404-407,共4页
Chinese Journal of Pharmaceutical Analysis
