摘要
目的:探讨贵阳腐霉最佳的保存方法,以延长其有效保质期。方法:以菌丝体生长速度和灭蚊毒力为指标,观察分别在不同培养基、温度、氧分压条件下保存1、2、3、4、5个月的贵阳腐霉的质量,总结对该真菌保存影响较大的因素。结果:观察发现,在水琼脂培养基上保存的菌丝体生长速度最快;在葵花籽提取物(SFE)培养基上保存的菌丝体灭蚊毒力最高;4℃是贵阳腐霉长期保存的最佳温度,在-25℃和-75℃条件下菌丝体不能存活;氧分压分别为100%、66.7%、33.3%及0时保存的菌丝体差别不大,但33.3%氧分压保存的菌丝体稍好。结论:本研究总结了贵阳腐霉保存的最佳条件,为其商业开发和工厂化生产提供了有价值的资料。
Objective： To explore optimal conditions for long-term storage of Pythium guiyangense Su. Methods： Hyphal growth speed and virulence to Culex quinquefaciatus larvae were used as indexes to evaluate quality of P. guiyangense. Factors affecting storage of the fungus including media, temper- ature, partial pressure of oxygen, were tested as storage conditions or condition combination. The fungus was stored under different conditions and tested in 1, 2, 3, 4, and 5 months after storage initiation. Results： Mycelia stored on water agar grew the fastest, while that stored on SFE agar media had the highest virulence; fungus stored under 4 ℃ showed the best quality during the whole observation course, while that stored under -25 ℃ and -75 ℃ did not survive; Partial pressure of oxygen of 33.3% could meliorate the storage condition. Conclusions： The optimal storage condition combinations might provide valuable information for commercial development and industry production of P. guiyan- gense.
出处
《贵阳医学院学报》
CAS
2013年第2期115-119,共5页
Journal of Guiyang Medical College
基金
国家自然科学基金(30760140)
贵州省攻关课题(黔科合NY字[2006]3040)
关键词
贵阳腐霉
长期保存
灭蚊毒力
菌丝体
Pythium guiyangense Su
long-term storage
virulence to mosquito
