摘要
为评价复合木霉制剂对黄连根腐病的防治效果,并揭示其防病机理,为黄连根腐病专用微生物农药的研发奠定基础,本试验将深绿木霉Trichoderma atroviride、长枝木霉T.longibrachiatum、钩状木霉T.hamatum、拟康宁木霉T.koningiopsis等4种木霉配制的复合制剂和尖镰孢Fusarium oxysporum以不同的方式分别施用于黄连,统计根腐病发生情况,检测黄连根部防御酶活性,用高通量测序分析根际土壤真菌群落结构。结果表明,复合木霉制剂对尖镰孢导致的根腐病具有明显预防效果;复合木霉制剂和尖镰孢分别接种黄连可提高SOD、POD、CAT、PAL、PPO等防御性酶活性,产生诱导抗性;而它们先后接种黄连可产生强化效应,从另一个途径提高植株抗病性。复合木霉制剂和尖镰孢都会降低真菌的数量、多样性,和某些真菌的相对丰度,而复合木霉制剂的抑菌作用更强烈,尤其能明显抑制尖镰孢、Ilyonectria sp.等病原真菌的生长,且能改善土壤真菌群落结构。木霉和尖镰孢都能在土壤中较长期定殖。可见,复合木霉制剂可以预防尖镰孢导致的黄连根腐病,防病机理包括诱导黄连植株产生抗性,接种后再遭受病原菌侵染产生的强化效应,优化土壤真菌群落结构,抑制土壤中病原菌等,且有效期较长。因此复合木霉制剂具有开发为微生物农药防治黄连根腐病的潜力。
To evaluate the effect of compound Trichoderma agent on Coptis chinensis root rot,investigate its mechanism and provide a theoretical basis for developing special microbial pesticides against this disease,compound Trichoderma agent,consisting of Trichoderma atroviride,T.longibrachiatum,T.hamatum and T.koningiopsis,and Fusarium oxysporum were applied to C.chinensis plants,respectively.The root rot symptoms were observed;the root defensive enzyme activities were determined,and fungal communities in the rhizosphere soil were analyzed by high-throughput sequencing.The results indicated that compound Trichoderma agent had obvious preventive effect on root rot caused by F.oxysporum.Compound Trichoderma agent and F.oxysporum,when applied respectively,could increase the activities of SOD,POD,CAT,PAL and PPO,inducing the plants’resistance,while strengthening effect was observed when compound Trichoderma agent and F.oxysporum were applied successively,improving plant resistance by other means.Both compound Trichoderma agent and F.oxysporum decreased the number and diversity of fungi and relative abundance of some fungi.Compared with F.oxysporum,compound Trichoderma agent had stronger fungistatic activity,inhibited pathogenic fungi such as F.oxysporum,Ilyonectria sp.,and improved soil fungal community structure.Both Trichoderma sp.and F.oxysporum could inhabit soil for a long time.Therefore,compound Trichoderma agent could prevent root rot disease caused by F.oxysporum.The disease-resistant mechanism included induced resistance,strengthening effect induced by pathogen after inoculation with compound Trichoderma agent,optimizing the fungal community,inhibiting soil pathogens,etc.Besides,compound Trichoderma agent had relatively long term of validity.In sum,compound Trichoderma agent had the potential as microbial pesticide against C.chinensis root rot disease.
作者
伍晓丽
王钰
刘飞
陈大霞
WU Xiaoli;WANG Yu;LIU Fei;CHEN Daxia(Institute of Medical Material Planting,Chongqing Academy of Chinese Materia Medica,Chongqing Sub-center of National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Chongqing Key Laboratory of Chinese Medicine Resources,Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia Medica,Chongqing 400065,China;Health Center of Chongqing Academy of Chinese Materia Medica,Chongqing 400065,China)
出处
《植物保护》
CAS
CSCD
北大核心
2024年第1期97-109,共13页
Plant Protection
基金
重庆市自然科学基金(cstc2019jcyj-msxmX0677)
重庆市基本科研业务费(cstc2019jxjl-jbky10004)
重庆市卫健委重点项目(ZY201801006)
重庆市现代农业产业技术体系创新团队(CQMAITS202309)
重庆英才计划创新领军人才项目(CQYC20200301170)。
关键词
复合木霉制剂
黄连根腐病
根际土壤
防御酶活性
真菌群落结构
compound Trichoderma agent
Coptis chinensis root rot disease
rhizosphere soil
defensive enzyme activities
fungal communities
