摘要
通过向液体培养基中添加无机硒,利用纳豆菌的发酵作用实现无机硒到有机硒的生物转化,并与纳豆激酶结合,得到硒纳豆激酶。最优发酵条件为:大豆蛋白胨2.5%,葡萄糖2%,Na2SeO310-6mol/L,MgSO40.05%,CaCl20.02%,K2HPO4/KH2PO40.2%/0.1%,培养基pH7,培养温度37℃,接种量2%,培养时间60h。所得硒纳豆激酶酶活达196.81U/mL(Folin-酚法),其SDS-PAGE凝胶电泳条带的硒含量为0.060μg/g,远高于不含硒发酵液对照样。
This study realized the biological transformation form inorganic selenium into organic selenium by liquid fermentation of the Bacillus natto,and obtained Selem＇Nattokinase. Optimal fermentation conditions were 2.5% soy peptone,2% glucose,10^-6mol/L Na2SeO3, 0.05% MGSO4,0.02% CACl2,0.2%/0.1% K2HPO4/KH2PO4, pH7.0,fermentation temperature 37℃ ,inoculum size 2% ,fermentation time 60h. Under these conditions,the activity of the Selem＇Nattokinase reached 196.81U/mL（Folin-phenol method）,and the selenium content of its SDS-PAGE protein band was 0.060μg/g.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第8期219-222,227,共5页
Science and Technology of Food Industry
关键词
纳豆激酶
硒
液体发酵
Nattokinase
Selenium
liquid fermentation
