摘要
从纳豆样品中分离筛选到一株产纳豆激酶的细菌,编号为BN-6。通过单因素实验和正交实验确定了液体发酵纳豆激酶的最佳条件:大豆蛋白胨1%、蔗糖2%、培养温度35℃,初始pH=8.0,培养时间24h。在最佳条件下,该菌产酶纤溶活力超过1000尿激酶单位/毫升发酵液。
A strain BN-6 of producing nattokinase was screened from natto. By single factor test and orthogonal,the best fermentation conditions of producing nattokinase were soybean tryptone 1%,sucrose 2 %, temperature 35 ℃, pH=8.0, 24 hours.In this conditions,the enzyme＇s fibrinolytic activity could reach more than 1 000 urokinase unit per millilitre broth.
出处
《食品研究与开发》
CAS
北大核心
2006年第4期27-29,26,共4页
Food Research and Development
关键词
纳豆芽抱杆菌
筛选
纳豆激酶
发酵条件
Bacillus natto
screened
nattokinase
fermentation conditions
