摘要
目的:研究纳豆杆菌营养体和芽孢之间的生物学特性。方法:营养消耗法制备纳豆杆菌芽孢悬液,以涂平板计数菌落法比较不同的温度、pH值、盐含量、体外模拟动物胃肠道环境对纳豆杆菌营养体及其芽孢的影响,并采用药敏试纸法检测两者的药物敏感性。结果:纳豆杆菌呈二次生长,其芽孢对温度、酸碱、盐和模拟胃肠道环境等不利环境的耐受性均明显强于营养体,但对抗菌药物(除杆菌肽外)较营养体敏感。结论:纳豆杆菌芽孢对不利因素的耐受性明显强于营养体,但对药物的敏感性较营养体强。
Objective： Comparison studies of biological characteristic features between Bacillus natto vegetative cells and spores. Methods： Natto Bacillus spores suspension liquid was prepared by using nutrition consumption method, comparied effect of different temperature, pH, salt content and simulated gastract-intestinal environment on Natto Bacillus vegetative cells and spores was compared by scribble the fiat counting bacterial colony method, check drug sensitivity between vegetative cells and spores by test paper. Results： Natto Bacillus appeared second growth, the endurance of spores are more robust than vegetative cells for temperature, pH, salt content and simulated gastract-intestinal environment, but more sensitivity to drug （besides Bacitracin） than vegetative cells. Conclusion： The endurance to unfavourable factors of Natto Bacillus spores are more robust than vegetative cells, but more sensitivity to drug than vegetative cells.
出处
《中国食品学报》
EI
CAS
CSCD
2007年第6期27-32,共6页
Journal of Chinese Institute Of Food Science and Technology
基金
江西省教育厅科研项目(赣教高字[2007]6号)
关键词
纳豆杆菌
营养体
芽孢
生物学特性
Natto Bacillus Vegetative cells Spores Biological characteristics
