摘要
为选育纳豆激酶(Nattokinase,NK)高活性菌株,从4种不同风味食品中分离得到26株菌株,通过酪蛋白平板初筛和液体发酵复筛,得到2株纳豆激酶高产菌株,利用分子生物学技术鉴定为2株枯草芽孢杆菌(Bacillus subtilis)。采用氯化锂-紫外与亚硝基胍(NTG)复合诱变的方式,最终获得1株纳豆激酶活性达到2338.01 U/mL的高产菌株SD3-3-6,该突变菌株纳豆激酶酶活相较于初始菌株SD3提高了1.93倍,是1株具有应用前景的高产菌株。
In order to select nattokinase high activity strains,26 strains were isolated from four different samples.Two nattokinase high-yield strains were screened through primary screening and rescreening,and identified as two Bacillus subtilis strains by molecular biology technology.A high-yield strain SD3-3-6 with nattokinase activity of 2338.01 U/mL was obtained by lithium chloride UV and NTG compound mutagenesis.The final activity of nattokinase of SD3-3-6 was 1.93 times higher than that of the original strain SD3.
作者
巩涛
魏传军
安明理
宁萌
周伏忠
GONG Tao;WEI Chuan-jun;AN Ming-li(Institute of Biology Co.,Ltd.,Henan Academy of Science,Zhengzhou,Henan 450008)
出处
《安徽农业科学》
CAS
2021年第15期159-161,175,共4页
Journal of Anhui Agricultural Sciences
基金
河南省科技攻关项目(212102310541)
河南省科学院科研项目(190305003、18JK16012)。
关键词
纳豆
纳豆激酶
多肽
复合诱变
芽孢杆菌
Natto
Nattokinase
Polypeptide
Mutagenesis
Bacillus subtilis
