摘要
目的:建立雷公藤根木质部与根皮的色谱指纹图谱。方法:采用高效液相色谱法分析。色谱柱:Inertsil ODS-SP(5μm,4.6 mm×250 mm);流动相:乙腈-水,二元梯度洗脱;检测波长:220 nm;流速:1.0 mL·min-1。测定10批药材根木质部、根皮的色谱图,建立标准指纹图谱。结果:雷公藤根木质部和根皮HPLC指纹图谱上的峰数和峰形基本相同,根木质部、根皮指纹图谱共有峰均有19个,但二者指纹图谱3、4号共有峰的峰位和峰形不同。结论:该分析方法的稳定性、精密度、重现性较好,为雷公藤药材根木质部与根皮的鉴别及质量控制提供了依据。
Objective： To develop a reliable high performance liquid chromatographic （HPLC）method for the fin- gerprint chromatogram study of the root xylems and barks from Tripterygium wilfordii Hook. F. , and compare the difference between them. Methods： HPLC separation was performed on an Inertsil ODS - SP column （5μm,4. 6 mm× 250 mm） and detected by ultraviolet absorbance at 220 nm. A mobile phase composed of acetonitrile and H20 was adopted at a flow rate of 1.0 mL ·min- 1 with gradient elution. Ten batches of sample were determined respec- tively. Results： The peak profile of the 19 components collected within 120 min made up the Tripterygium wilfordii Hook. F. with universal features. The No. 3 and No. 4 common peaks in the fingerprints of the root xylems and barks were different. Conclusion： The fingerprint chromatograms had a good precision, stability, and reproducibility. The method is suitable for differentiation of the root xylems and barks from Tripterygium wilfordii Hook. F. , the present study provided a new evidence for the criterion of preparation process.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2013年第3期419-423,共5页
Chinese Journal of Pharmaceutical Analysis
基金
福建省自然科学基金项目(2010J01024)
