摘要
目的探索建立阳春砂、绿壳砂和海南砂的高效液相色谱指纹图谱,并比较三者的差异。方法采用反相高效液相色谱法,色谱柱为Agilent ZORBAX SB-Aq C18分析柱(4.6 mm×250 mm,5μm),流动相为甲醇(A)-0.05%甲酸溶液(B),二元梯度洗脱(0 min,5%A;5 min,5%A→15%A;10 min,15%A→26%A;20 min,26%A→40%A;45min,40%A→70%A;58min,70%A→100%A,63 min,100%A),检测波长为263 nm,柱温30℃,流速1.0 m L·min-1。采用中药色谱指纹图谱相似度评价系统(2012.0版本)和SPSS21.0对谱图进行相似度分析和聚类分析。结果从对照指纹图谱和聚类分析结果看,阳春砂与海南砂共有模式相似,两者与绿壳砂差别明显,分别建立对照指纹图谱,各有29和25个共有峰。结论首次建立了3种砂仁非挥发性成分HPLC指纹图谱,为不同品种砂仁的鉴别和质量控制提供参考。
OBJECTIVE To establish the HPLC fingerprints of Amomum villosum Lour.,Amomum villosum Lour.var.xanthioides T.L.Wu et Senjen and Amomum longiligulare T.L.Wu and find their differences.METHODS The samples were extracted with 75% ethanol aqueous and then analysis was carried out on an Agilent ZORBAX SB-Aq C_（18） column with the mobile phase consisting of methanol（A） and 0.05% formic acid solution（B）.Gradient elution（0 min,5% A;5 min,5% A →15% A;10 min,15% A→26% A;20 min,26% A→40% A;45 min,40% A→70% A;58 min,70% A→100% A,63 min,100% A） was carried out at the flow rate of 1.0 m L·min^（-1）.The column temperature was maintained at 30 ℃,and the detection wavelength was set at263 nm.The software ＂Similarity Evaluation System for Chromatographic Fingerprint of TCMs（Version 2012.0） ＂was employed to generate the mean chromatogras and carry out the similarity analysis of the samples.SPSS21.0 was employed to carry out the cluster analysis.RESULTS The HPLC fingerprints of the three varieties were different according to fingerprinting and cluster analysis.Amomum villosum Lour.var.xanthioides T.L.Wu et Senjen was obvilously differernt from Amomum villosum Lour.and Amomum longiligulare T.L.Wu.There were 25 common peaks in the former HPLC fingerprint and 29 common peaks in the latter.CONCLUSION The HPLC fingerprints of three kinds of Amomum villasums were set up for the first time and they provide reference for the identification and quality control of Amomum villosum Lour.,Amomum villosum Lour.var.xanthioides T.L.Wu et Senjen,and Amomum longiligulare T.L.Wu.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2016年第12期1039-1043,共5页
Chinese Pharmaceutical Journal
基金
深圳市基础研究计划基础研究项目(JCYJ20120830153011458)
关键词
高效液相色谱法
指纹图谱
阳春砂
绿壳砂
海南砂
HPLC
fingerprint
Amomum villosum Lour
Amomum villosum Lour.var.xanthioides T.L.Wu et Senjen
Amomum longiligulare T.L.Wu
