摘要
本文对自制的改性壳聚糖-明胶交联膜(MC-Gel)与壳聚糖-明胶交联膜(CS-Gel)的理化性能、生物相容性进行测定与评价,探讨其用于角膜组织修复与重建的可行性。采用紫外分光光度计测定了交联膜的透光率及渗透性,结果显示两组交联膜的透光率超过90%,且对葡萄糖、色氨酸与NaCl的渗透率均接近或优于人角膜的渗透率,可满足角膜修复对透光率及营养物质透过的要求。同时,采用MTT法测定了人角膜上皮细胞(HCEC)在两组膜上的生长活性,并评价了两组交联膜在兔眼角膜内的组织相容性。实验结果表明,MC-Gel能较好地支持HCEC在其上增殖,且植入兔眼角膜后无明显炎症反应,术后4个月材料全部降解。因此,该交联膜具有良好的理化性能与眼组织相容性,有望成为一种良好的角膜修复材料。
In order to investigate the feasibility of the modified chitosan-gelatin crosslinked membrane （MC-Gel） and chitosan-gelatin crosslinked membrane （CS-Gel） to be a potential biomaterial for corneal regeneration, we evaluated their physicochemical properties and intraocular biocompatibility in this study. White light transmission and permea- bility of these membranes were detected. Results showed that white light transmission of both membranes was above 90% at 500 nm, which was similar to that of human cornea. The glucose, tryptophan and NaC1 permeability of MC- Gel membrane and CS-Gel membrane was better than or similar to those of human cornea. The methylthiazol tetrazo- lium （MTT） assay was used to assess cell viability and proliferation. Also, interlamellar corneal transplantation was carried out to evaluate ophthalmic biocompatibility of MC-Gel membrane and CS-Gel membrane. Results indicated that MC-Gel membranes could support the proliferation of HCEC and displayed good intraocular biocompatibility when implanted into rabbits. No severe inflammatory reaction occurred after transplantation and the implanted MC- Gel membrane degraded completely 16 weeks post-operation. Due to its good physicochemical properties and biocom- patibility, MC-Gel membrane could be a promising candidate material for corneal regeneration.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2013年第1期170-175,共6页
Journal of Biomedical Engineering
基金
"十二五"国家科技支撑计划项目资助(2012BAI17B02)
国家自然科学基金资助项目(50803018
51273072)
国家863计划项目资助(2011AA030105)
广东省重大科技专项项目资助(2010A080407008
2012A080203010)
广东省教育部产学研结合项目资助(2010B090400416)
眼科学国家重点实验室创新基金资助项目(2011C05)
关键词
壳聚糖
明胶
渗透性
生物相容性
层间角膜移植
Chitosan
Gelatin
Permeability
Biocompatibility
Interlamellar corneal transplantation
