摘要
目的 探讨葡多酚 (GPC)对放射性DNA损伤的防护作用。方法 体内实验 :小鼠口服GPC ,用60 Co γ射线一次性全身照射 ,检测脾细胞DNA含量 ;体外实验 :将加入GPC的人静脉血用60 Co γ射线照射 ,在照射后 1、30、6 0min测定白细胞DNA含量。结果 GPC高、中、低 3个剂量组小鼠脾细胞DNA含量分别比阳性对照组升高 18 76 %、15 74%和 5 0 6 % ,照射后 1min白细胞DNA含量分别比阳性对照组高出 17 6 %、11 1%、3 9% ,6 0min时高出 2 1 3%、13 8%、5 3% ,差异均有显著性意义。结论 葡多酚对放射性DNA损伤有良好防护作用 。
Objective To study the protective effect of grape procyanidins (GPC) on the irradiated DNA damage Method In vivo study: After orally administration of GPC, mice in different groups were exposed to whole body 60 Co γ rays at a dose of 2 0 Gy and the fluorospectrometry method was used to rapidly detect the DNA strand breaks of mouse spleen cells In vitro study: Human venous blood with different concentrations of GPC was exposed to 60 Co γ rays at a dose of 1 0 Gy, then WBC DNA content was measured after 1, 30 and 60 min exposure, respectively Results In the three treated groups (500, 250 and 50 mg/kg of procyanidins), DNA content of mouse spleen cells was 18 76%, 15 74% and 5 06% more than that of the positive controls respectively DNA content of human WBC was higher than the positive controls by 17 6%, 11 1% and 3 9% increase respectively one minute after irradiation and 21 3%, 13 8% and 5 3% increase respectively 60 min after irradiation These differences were all statistically significant Conclusion GPC has a protective effect on DNA damage caused by irradiation, and is helpful to DNA repair
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2000年第3期131-133,共3页
Chinese Journal of Preventive Medicine
基金
国家自然科学基金!( 3 9870 678)
