摘要
目的:建立刺五加及短梗五加根皮刺五加苷B、刺五加苷E及异嗪皮啶的高效液相测定方法,测定刺五加及短梗五加中上述成分的含量,对比研究刺五加及短梗五加根中的成分含量差异。方法:采用高效液相色谱法,Agilent TC-C18色谱柱(150 mm×4.6 mm,5μm),以甲醇(A)-水(B)为流动相,梯度洗脱:(0～15 min:20A;15～20 min:21A;20～35 min:30A),流速0.8 mL.min-1,柱温30℃,检测波长210 nm。结果:样品进样量在各自浓度范围内呈良好的线性关系,加样回收率分别为99.1%、97.3%和99.9%,RSD为0.7%和1.3%和0.6%。结论:本法操作简便,分离效果好,可用于刺五加及短梗五加根皮刺五加苷B、刺五加苷E及异嗪皮啶的含量测定。
To establish the determination of eleutheroside B, E and Isofraxidin in Acanthopanax senticosus （ Rupr. et Maxim. ） Harms and Acanthopanax sessilifloms （ Rupr. et Maxim. ） Seem. root coat by HPLC, to determinate the content of eleutheroside B, E and Isofraxidin in Acanthopanax senticosus （ Rupr. et Maxim. ） Harms and Acanthopanax sessilifloms （ Rupr. et Maxim. ） Seem. root coat, to find the different between them. Method： HPLC method used to obtain chromatograms column （150 mm ×4.6 mm, 5 μm）, motion phase methanol （A） - water （ B）, gradient elution （0 ～15 min ： 20A ; 15～20 min ：21A;20 -35 min：30A）, velocity of flow 0.8 mL · min^-1, column temperature 30 ℃ , determinating wave length 210 nm. Results： The three compounds in their respective peak areas had good line arities and the average recovery rates were 99.1%, 97.3% and 99.9%, RSD was 0.7% ,1.3% and 0.6%. Conclusion： This method was simple and the results were satisfactory, which can be used for the content of eleutheroside B, E and Isofraxidin in Acanthopanax senticosus （ Rupr. et Maxim. ） Harms and Acanthopanax sessilifloms （Rupr. et Maxim. ）Seem. root coat.
出处
《中国野生植物资源》
2012年第6期37-39,共3页
Chinese Wild Plant Resources
