摘要
目的:建立眼明熏洗液(金银花、川芎、一枝蒿)的质量控制方法。方法:采用薄层色谱法对金银花、川芎进行定性鉴别,采用高效液相色谱法测定眼明熏洗液中绿原酸、阿魏酸含量,采用紫外分光光度法测定眼明熏洗液中总黄酮的含量。结果:薄层色谱斑点清晰,阴性对照无干扰。绿原酸在0.089～0.799 g·L-1线性关系良好,r=0.999 5(n=5),平均回收率为100.21%,RSD 1.06%(n=6);阿魏酸在7.434～37.17 mg·L-1线性关系良好,r=0.999 9(n=5),平均回收率为99.91%,RSD1.19%(n=6);芦丁在0.008～0.048 g·L-1线性关系良好,r=0.998 9(n=5),平均回收率为99.50%,RSD 0.87%(n=6)。结论:建立的TLC,HPLC,UV方法专属性强、重复性好,可用于眼明熏洗液的质量控制。
Objective： To establish the methods for quality control of Yanmingxunxi Lotion （Lonicerae Japonicae Flos, Chuanxiong Rhizoma, Herba Artemisiae rupestris）. Method： Lonicerae Japonicae Flos, Chuanxiong Rhizoma were identified by TLC. HPLC methods for the determination of chlorogenic acid, ferulic acid in Yanmingxunxi Lotion were established. UV method for the determination of total flavones was established. Result： The spots on the TLC plate were clear. The negative control sample didn＇t disturb. Chlorogenic acid showed good linearity in the range of 0. 089-0. 799 g ·L^-1, r = 0. 999 5 （ n = 5 ）. The average recovery was 100.21% , RSD was 1.06% （n = 6）. Ferulic acid showed good linearity in the range of 7. 434-37.17 rag. L-l, r =0. 999 9 （n =5）. The average recovery was 99.91% , RSD was 1.19% （n =6）. Rutin showed good linearity in the range of 0.008-0.048 mg .mL^-1, r =0.998 9 （n =5）. The average recovery was 99.50% , RSD was 0.87% （n =6）. Conclusion： The methods are simple, rapid, accurate and can be used for the quality control of Yanmingxunxi Lotion.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第23期123-127,共5页
Chinese Journal of Experimental Traditional Medical Formulae
