摘要
本文采用70%乙醇回流提取4个小时,再用流动相为乙腈-0.4%磷酸溶液(15:85),检测波长为327nm的高效液相色谱进行分析。结果重复性RSD为0.38%,平均加标回收率分别为97.7%、104.2%、97.7%,RSD<2%,该方法简便,易于操作,结果准确可靠。
Used 70% alcohol to reflux and distill for four hours,then used mobile phase of acetonitrile-0.4% phosphoric acid （15：85） and 327nm detection wavelength of high performance liquid chromatography to analyse. The result was that RSD of repeatability was 0.38% and average recovery rate were 97.7% 、104.2% 、97.7% respectively,RSD2% . The method was simple,easy to operate,accurate and reliable.
出处
《福建分析测试》
CAS
2010年第3期79-82,共4页
Fujian Analysis & Testing
关键词
高效液相色谱
金盏银盘
绿原酸
high performance liquid chromatography
railway beggarticks herb
chlorogenic acid
