摘要
目的筛选逍遥散抗抑郁有效部位X7、X6的最佳提取工艺。方法依据有效部位X7、X6的化学性质及其工艺操作可行性,设计一系列提取工艺,分别得到不同的提取物,以小鼠行为绝望模型(小鼠悬尾实验)评价各提取物的抗抑郁药效。将工艺操作可行性良好、抗抑郁效果接近原有效部位的提取物,运用HPLC进行化学指纹图谱的研究,从而与原有效部位进行指纹图谱的相似性对比分析,最终筛选出有效部位的最佳提取工艺。结果原有提取工艺所得有效部位抗抑郁活性良好,接近阳性药文拉法辛。工艺3与工艺5操作可行性更好,所得提取物其抗抑郁药效接近原有效部位,且工艺5所得提取物与原有效部位化学成分基本相同。结论工艺5(原有提取工艺中的萃取改为硅藻土拌匀后石油醚提取)可代替原有提取工艺,作为逍遥散抗抑郁有效部位X7、X6的最佳提取工艺。
Objective To screen the best extraction process of active fractions from Xiaoyaosan. Methods A series of similar extrac-tion processes with the original extraction process were designed by changing the solvent and process. The commonly used mouse tail suspension test （TST） model was selected to investigate the antidepressant effect of extracts. Furthermore ,HPLC was applied to estab-lish the fingerprints of the extracts possessing significant efficacy. The fingerprints were evaluated to screen the optimum extraction method of active fractions. Results The locomotor activity of mice in open field test （OFF） was not affected by the medicine treat-ment. The effect of extracts from the process 0 （ original extraction process） in the mouse TST model was the closest to the positive drug. The process 3 and process 5 were easy to conduct and the effects were similar to that of process 0. In addition, the chemical com-positions of extracts from process 0 and process 5 were consistent. Conclusion The process 5, collected by ethanol and extracted by petroleum ether after mixed with bergmeal, is the optimum extraction method for active fractions from Xiaoyaosan instead of the original extraction process.
出处
《山西医科大学学报》
CAS
2012年第11期825-829,共5页
Journal of Shanxi Medical University
基金
科技部国际合作基金资助项目(2008DFA30430)
国家自然科学基金资助项目(30901960)
关键词
逍遥散
抗抑郁
有效部位
提取工艺
Xiaoyaosan
anti-depression
active fractions
extraction process
