摘要
目的:为了阐明逍遥散乙醇提取物的乙酸乙酯萃取物(XYE-E)的活性成分与小鼠悬尾试验(TST)和强迫游泳试验(FST)不动时间的相关性,表征逍遥散的抗抑郁药效物质基础。方法:采用灰色关联分析、相关性分析以及强迫引入回归分析方法将1H-NMR图谱中各组分特征峰的相对峰面积与小鼠TST和FST不动时间数据相关联。结果:逍遥散提取物1H-NMR图谱中共指认出14种化学成分,其中确定与药效有较强相关性的化学物质有8个,分别为柴胡皂苷a,柴胡皂苷c,柴胡皂苷E,柴胡皂苷F,柴胡皂苷G,柴胡皂苷b2,白术内酯Ⅰ和白术内酯Ⅱ。结论:本研究发现与XYE-E抗抑郁药效密切相关的物质主要是柴胡皂苷。
The article is aimed to find the correlation between bioactive components of XYE-E and the antidepressant efficacy, by analyzing the immovability time in tail suspension test （TST） and forced swimming test （FST）. Using the method of gray relational analysis, correlation analysis and regression analysis, relating the peak area of each common peak of 1H-NMR spectra with the immovability time in TST or FST, we found that there were total 14 chemical components identified in the 1H-NMR spectrum of XYE-E. Among them, 8 compounds, including saikosaponin a, saikosaponin c, saikosaponin E, saikosaponin F, saikosaponin G, saikosaponin b2, atractylenolide I and atractylenolide II, had significant correlation with antidepressant efficacy.
出处
《世界科学技术-中医药现代化》
2015年第3期563-568,共6页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金委面上项目(81102833):基于c AMP-PKA-CREB-BDNF信号转导通路的逍遥散抗抑郁有效部位作用机制研究
负责人:田俊生
国家科技部"重大新药创制"科技重大专项课题(2012ZX09103201-035):复方柴归抗郁组分新药候选药物研究
负责人:秦雪梅
国家国际科技合作计划项目(2011DFA32630):从逍遥散古方中研发抗抑郁现代中药
负责人:秦雪梅
关键词
逍遥散乙醇提取物的乙酸乙酯萃取物
核磁共振
谱效关系
悬尾试验
强迫游泳试验
Ethyl acetate fraction in ethanol extract efficacy, tail suspension test, forced swimming test of Xiaoyaosan, 1H-NMR
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