摘要
目的:观察甘松95%乙醇提取物及其不同极性部位对动物行为绝望模型的影响。方法:ICR小鼠,雌雄兼用,分为两批,每批均设正常组和5个治疗组。第1批,治疗组连续10 d,ig给予甘松95%乙醇提取物(14.7,29.3,48.8,146.5 mg.kg-1)和阳性药氢溴酸西酞普兰(10.0 mg.kg-1);第2批,治疗组连续10d,ig给予甘松的4个极性部位(均为48.8 mg.kg-1)和阳性药氢溴酸西酞普兰(10.0 mg.kg-1)。末次给药后1h,用小鼠悬尾及强迫游泳实验进行行为学观察。结果:甘松95%乙醇提取物(29.3,48.8,146.5 mg.kg-1)能显著降低小鼠悬尾不动时间及强迫游泳不动时间(P<0.05)。在甘松4个极性部位中,乙酸乙酯部位与正丁醇部位均能显著降低小鼠悬尾不动时间及强迫游泳不动时间(P<0.05)。结论:甘松95%乙醇提取物有一定的抗实验性抑郁的作用,其活性部位可能为乙酸乙酯部位与正丁醇部位。
Objective：To observe the effect of ethanol extract and its four fractions from Nardostachyos Radix et Rhizoma on the despair animal models of depression.Method： ICR mice were divided into two batches,then every batch divided into control and five treatment groups.In the first batch,treatment groups were received ethanol extract at dose of 14.7,29.3,48.8,146.5 mg·kg-1and citalopram hydrobromide at 10.0 mg·kg-1for ten days.In the second batch,treatment groups were received four fractions at dose of 48.8 mg·kg-1 and citalopram hydrobromide at 10.0 mg·kg-1for ten days.1 h after the last administration,forced swimming test（FST） and tail suspension test（TST） were conducted for evaluation of behavior.Result： 95% ethanol extract（29.3,48.8,146.5 mg·kg-1） could significantly reduce the duration of murine immobility（P0.05）.Among four fractions n-butanol fraction and ethyl acetate fraction could significantly reduce the duration of murine immobility（P0.05）,and ethyl acetate fraction was better than n-butanol fraction.Conclusion： Nardostachyos Radix et Rhizoma possesses antidepressant effect,n-butanol fraction and ethyl acetate fraction could be the effective fractions.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第7期205-207,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
"重大新药创制"科技重大专项(2009ZX09103-381)
国家科技基础条件平台工作项目(2005DKA21000)
关键词
甘松
抗抑郁
悬尾实验
强迫游泳实验
Nardostachyos Radix et Rhizoma
antidepressant
tail suspension test
forced swimming test
