摘要
将黄孢原毛平革真菌 (Phanerochaetechrysosporium ,PC)真核细胞与YZI土著细菌原核细胞的原生质体进行融合 ,构建而成Fhh融合子 ;再将Fhh与酵母 (Saccharomycescerevisiae ,Y99)真菌真核细胞的原生质体进行融合 ,构建而成Fhhh融合子 .Fhh与Fhhh是跨真核原核两界细胞的两株融合子 ,对制霉菌素和链霉素同时存在抗性 ,孢子和菌丝形态均不同于PC菌 .在精对苯二甲酸生产废水中降解反应 6h ,Fhhh的比增长率 μ和比降解率q均处最高水平 ,分别是 0 12 6 5h- 1 和 0 .3348h- 1 ,分别为PC、YZI、Y99三亲株菌的 5～ 10倍和 11～ 19倍 .研究结果对于提高PTA废水处理系统的处理效率 ,降低处理费用 ,有着重要的意义 .
Fhh is the first fusant between Phanerchaete chrysosporium and the native bacterium YZI.Fhhh is the second fusant between Fhh and Saccharomyces cerevisiae . Both Fhh and Fhhh were obtained through inter kingdom protoplast fusions in this research. The two fusant strains had the resistance to the antibiotics of streptomycin and nystatin, but the three parental strains could only grow on the medium with one of the antibiotics. The morphologies of the two fusant strain spore and mycelium were different from those of the parental strains. The values of the two kinetic parameters of μ and q for Fhhh in pure terephthalic acid (PTA) wastewater in 6?h reaction were both at the highest levels in the five strains, which were 0.126?5?h -1 and 0.334?8?h -1 . The results suggested that the technique of inter kingdom protoplast fusion might offer ideal new strains for increasing purification rate and decreasing operation cost.
出处
《南京大学学报（自然科学版）》
CAS
CSCD
2000年第3期312-316,共5页
Journal of Nanjing University（Natural Science）
基金
国家自然科学基金项目
江苏省自然科学基金项目
关键词
精对苯二甲酸
降解
废水处理
PC菌
微生物
protoplast fusion
pure terephthalic acid wastewater
kinetic parameter
