摘要
从扬子石油化工责任有限公司废水处理装置的污泥池中分离、筛选得到了一株高效降解对苯二甲酸(PTA)的菌株,PA18.采用PCR扩增获得该菌16SrDNA片段,核苷酸序列分析结果表明:该菌株的16SrDNA的核苷酸序列与多株假单胞菌(Pseudomonassp.)的同源性为99%,在细菌系统发育分类学上,该菌株属于假单胞属.在温度为37℃、pH7.0、OD660nm2.0条件下,反应24h,此菌对PTA的降解率大于95%.同时对于实际的PTA废水也有很好的处理效果,CODCr去除率高达85%.在好氧降解作用下,PTA降解的主要中间物被确定为原儿茶酸.
A strain PA-18 was isolated from the active sludge of p-phthalic acid （PTA） wastewater treatment in Yangtze Petrochemical Company. The 16SrDNA fragment was amplified from strain PA-18 by PCR, and the 16SrDNA sequence analysis indicated that strain PA-18 shared 99% 16SrDNA sequence homology with Pseudomonas sp.. In the phylogenetic framework of bacterial classification, PA-18 belongs to Pseudomonas sp.. Under the condition of temperature 37℃, pH 7.0 and OD660nm 2.0, the PTA decomposition rate of this bacterial strain was beyond 95% after 24 h. Meanwhile, this strain also could treat PTA wastewater with CODCr removal of 85%. p-Phthalic acid was degraded by the strain PA-18 through the intermediate formation of protocatechuic acid in the aerobic state.
出处
《化工学报》
EI
CAS
CSCD
北大核心
2006年第8期1968-1973,共6页
CIESC Journal
基金
国家"十五"科技攻关项目(2004BA313B16)
国家自然科学基金项目(20376034)
江苏省社会发展项目(BS2003030
BS2005053).~~
