摘要
研究了采用原生质体电融合技术构建高效的重金属去除菌;对影响电融合效率的几个参数,以及融合子的生长条件、除铬性能和遗传稳定性等方面进行了考察;确定了进行电融合的最佳条件,并选出 1株最好的融合株R32. 实验结果表明:R32不论是在处理低浓度还是高浓度的铬液时,其去除率和还原率都明显高于 2株亲本菌,处理低浓度含铬废水时,去除率和还原率可达到 100%;处理高浓度含铬废水(200mgL-1 )时,还原率仍可达 50%以上. 经过多次传代后,R32的除铬能力保持稳定. 当投菌量>10gL-1 (湿重)时,其去除率和还原率都在 80%以上. 正交实验结果显示,pH和Cu2+浓度对R32的生长影响都不大,这些特点都有利于R32在实际含铬废水处理中的应用. 图 4表 3参
Protoplast-electricity-induced fusion was used to construct high-efficiency heavy metal removal strains. The factors that affected electricity-induced fusion, growth conditions, heavy metal removal capacity and genetic stability of the fusant were studied. Optimum requirements of electricity-induced fusion were confirmed and a fusant strain was selected. The results showed that the reduction and removal rate of R 32 in Cr-solutions of both low and high concentrations were better than those by original strains. Cr(VI) was reduced and removed at a rate of 100% when Cr 6+ concentration was low, while more than 50% of Cr(VI) were reduced when Cr 6+ concentration was high(200 mg L -1). The removal capacity of R 32 could be past from generation to generation. When R 32 dosage was 10 g L -1 (wet weight), the removal and reduction rates of R 32 were both more than 80%. The results of orthogonal experiments showed that pH and Cu 2+ concentration had rather low effect on the growth of R 32. All of these characteristics would contribute to the application of R 32 in the treatment of Cr-containing wastewater. Fig 4, Tab 3, Ref 14
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2005年第1期78-81,共4页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金资助项目 ( 50278040 )
广东省科技计划项目(2003C32103)资助项目~~
关键词
原生质体
电诱导融合
重金属
热带假丝酵母
解脂假丝酵母
protoplast
electricity-induced fusion
heavy metal
Candida tropicalis
Candida lipolytica
