摘要
目的:研究石榴皮鞣质对糖尿病模型大鼠肾脏氧化应激反应的影响。方法:将50只SD大鼠单次腹腔注射链脲佐菌素(75mg.kg-1)诱发实验性糖尿病,模型复制成功后随机分为模型(等容生理盐水)、维生素E(20mg.kg-1)与石榴皮鞣质高、中、低剂量(200、100、50mg.kg-1)组,另将10只SD大鼠作为正常对照组。灌胃给药,每天1次,连续21d。末次给药后观察大鼠日饮水量、日进食量,测定血糖(GLU)、血肌酐(Scr)、尿素(Urea),取一侧肾脏匀浆后测肾皮质超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH)活性、丙二醛(MDA)含量,另一侧肾脏做病理形态学检查。结果:与模型组比较,石榴皮鞣质高、中、低剂量组日饮水量、日进食量显著减少(P<0.01或P<0.05),GLU、Scr、Urea水平显著下降(P<0.01),SOD、GSH-PX活性显著减弱(P<0.01),MDA含量显著下降(P<0.01),肾组织病理学损害明显改善。结论:石榴皮鞣质能减轻糖尿病大鼠肾皮质氧化应激反应,有保护肾脏和延缓糖尿病肾病发生的作用。
OBJECTIVE：To study the effects of Punica granatum tannin on oxidative stress of kidney in diabetic rats.METHODS：50 SD rats was given a single injection of streptozotocin（STZ 75 mg.kg-1）to establish diabetic model.The model rats were randomly divided into model group（constant volume of normal saline）,vitamin E group（20 mg.kg-1）,P.granatum tannin low-dose,middle-dose and high-dose groups（200,100 and 50 mg.kg-1）with other 10 SD rats as normal control group.All rats were given medicine intragastrically once a day for 21 days.Biochemical indicators,the activities of SOD and GSH-PX and MDA content in the renal tissue were measured after the last administration.The pathological changes of kidney on the other side were also detected.RESULTS：Compare with model group,daily water intake,daily food intake,blood glusose of rats,and the levels of serum creatinine and urea nitrogen and MDA content in P.granatum tannin low-dose,middle-dose and high-dose groups decreased significantly（P〈0.01）,and SOD and GSH-PX activities increased significantly.The pathological injury of renal tissue was improved significantly.CONCLUSION：P.granatum tannin can relieve the oxidative stress of renal cortex to play protective effect on kidney and delay the development of diabetic nephropathy.
出处
《中国药房》
CAS
CSCD
2012年第39期3662-3664,共3页
China Pharmacy
基金
湖北省宜昌市科技局立项课题资助
关键词
石榴皮鞣质
糖尿病
肾脏
氧化应激
Punica granatum tannin
Diabetic
Kidney
Oxidative stress
