摘要
利用前期双重抑制PCR技术分离得到的10个鸭微卫星标记,分析了13个鸭品种的遗传多样性。利用等位基因频率计算各群体的平均遗传杂合度(H)、多态信息含量(PIC)和群体间的Nei氏遗传距离(DA)等遗传参数,并用类平均法进行了聚类分析。结果表明:10个微卫星座位中有7个具有多态,可作为有效的遗传标记用于各品种的遗传多样性和系统发生关系分析。7个微卫星座位在13个品种中共检测到51个等位基因;每个座位的等位基因数为5～12个,平均多态信息含量为0.402 3～0.601 5;各群体平均杂合度为0.407 0～0.707 0,说明各群体的遗传多样性较为丰富。采用DA/UPGMA法聚类分析,13个鸭群体聚为3类:Ⅰ类为8个家鸭品种和樱桃谷鸭;Ⅱ类为野鸭类;Ⅲ类为番鸭独成一类。结果提示:各类鸭群体间的聚类关系与其来源、分化、地理分布基本一致,而且绿头野鸭和斑嘴鸭在家鸭品种形成过程中均作出了贡献。
In this research, 10 microsatellite DNA markers in duck previously isolated through dual-suppression-PCR technique were used to analyze the genetic diversity of 13 duck breeds. The genetic parameters of mean heterozygosity （H） , polymorphism information content （PIC） , and Nei＇s genetic distance （DA） were calculated for each breed. The UP-GMA clustering dendrogram was drawn as well. The results showed that 7 microsatellite polymorphic loci were identified, and were regarded as effective markers to analyze genetic diversity and phylogenic relationship among duck breeds. Fifty one alleles were detected in all the loci, and alleles in each locus ranged from 5 to 12. The mean PIC value was between 0.402 3 and 0. 601 5, and the mean heterozygosity was between 0. 407 0 and 0. 707 0, indicating that the genetic diversity of the 13 breeds was very abundant. UPGMA dendrogram constructed based on DA genetic distance revealed that the 13 duck breeds were clustered into 3 groups. In the cluste- ring tree, 8 indigenous duck breeds in China and Cherry Valley ducks were the first group, wild ducks were the second group, and Muscovy ducks had its own branch. The result of UPGMA was consistent with the genesis, dif-ferentiation and location of the duck populations. Both An- as platyryhynchos and Anas peocilorhycha contributed to domestic duck evolution.
出处
《江苏农业学报》
CSCD
北大核心
2012年第4期809-814,共6页
Jiangsu Journal of Agricultural Sciences
基金
国家科技攻关项目(2002BA514A-9-2-6)
江苏省科技支撑计划(农业)项目(BE2011465)
江苏牧院院级课题(QN201002)
关键词
微卫星
鸭
遗传多样性
microsateilite marker
duck
genetic diversity
