摘要
【目的】了解中国部分地方鸭品种的群体遗传结构和遗传分化.【方法】使用荧光标记的微卫星引物和ABI-3730XL型DNA测序仪器,对我国6个地方鸭品种(180只)在16个微卫星位点上的等位基因进行STR分型测序,利用Structure和Fstat软件对群体结构和遗传分化进行分析.【结果】16个微卫星位点共检测到141个等位基因,具有高度多态性;F-统计量结果显示群体间存在显著的遗传分化;Structure程序成功构建出了6个鸭品种的遗传分化结构图;Structure程序构建的Neighbor Joining(NJ)图将‘润州凤头白鸭’‘于田麻鸭’聚为一类,‘北京鸭’与‘樱桃谷鸭’‘吉安红毛鸭’聚为一类.【结论】通过16个多态性较好的微卫星位点对中国6个地方鸭品种进行了遗传结构性和遗传分化研究,为获取我国地方鸭品种间的遗传信息和遗传关系提供更准确的依据.
【Objective】To understand the genetic structure and genetic differentiation of local duck species in China.【Method】In this study,the fluorescent-labeled microsatellite primers were used for STR typing of alleles in 16 microsatellite loci in 6 duck cultivars(180 heads)preserved in China by ABI-3730XL DNA sequencing platform.Population structure and genetic differentiation were analyzed using Structure and Fstat software.【Result】A total of 141 alleles were detected in 16 microsatellite loci with high polymorphism.The F-statistics showed significant genetic differentiation among populations.The Structure program successfully constructed the genetic differentiation structure of 6 duck breeds.The Neighbor Joining(NJ)map constructed by the Structure program showed that‘Runzhou White Crested Duck’and‘Yutian Ma Duck’were clustered together,and‘Beijing Duck’‘Cherry Valley Duck’and‘Ji'an Red-haired Duck’were clustered together.【Conclusion】In this study,16 microsatellite loci with abundant polymorphism were used to study the genetic structure and genetic differentiation of 6 local ducks in China,which provided more accurate basis for obtaining the genetic information and genetic relationship among local ducks in China.
作者
王统苗
张莘
陈博雯
常国斌
白皓
江勇
张扬
王志秀
陈国宏
WANG Tong-miao;ZHANG Xin;CHENG Bo-wen;CHANG Guo-bin;BAI Hao;JIANG Yong;ZHANG Yang;WANG Zhi-xiu;CHEN Guo-hong(College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China;Joint International Research Laboratory of Agriculture and Agri-Product Safety,Ministry of Education of China,Institutes of Agricultural Science and Technology Development,Yangzhou University,Yangzhou 225009,China)
出处
《甘肃农业大学学报》
CAS
CSCD
北大核心
2019年第6期24-29,共6页
Journal of Gansu Agricultural University
基金
江苏现代农业(水禽)产业技术体系(JATS[2018]301)
江苏省农业科技自主创新资金项目(CX(18)1004)
