摘要
通过四个微卫星位点对伊犁马进行了遗传检测,用8%非变性聚丙烯酰胺凝胶电泳分离PCR扩增产物。统计该群体的等位基因的组成、等位基因数。利用等位基因频率计算出多态信息含量、杂合度及其平均值。结果表明四个微卫星位点在伊犁马中的平均多态信息含量为0.697 3,属高度多态,可作为有效的遗传标记用于伊犁马遗传多样性的分析。
The genetic polymorphisms of 4 microsatellite were detected in lli horse . PCR amplification production were separated by 8% nondenaturing PAGE . Number of aUeles and alleles frequency were counted . According to alleles frequencies of 4 microsatellite Locus, Polymorphic information contents （PIC ）, heterozygosity （ H ） and .their mean values were calculatied. The average PIC of 4 microsateilite Locus were 0.697 3 , which showed these microsateilite Locus were effective markers for genetic analysis on ni horse.
出处
《新疆农业科学》
CAS
CSCD
2008年第3期575-578,共4页
Xinjiang Agricultural Sciences
基金
国家科技部国际科技合作重点项目“乳用马品种引进及杂交改良初步试验研究”(2005DFA30760)
新疆维吾尔自治区重点攻关项目“引进乳用马品种开展杂交改良试验研究”(200541101)
