摘要
目的:建立HPLC测定柴芩软胶囊中葛根素的含量的方法。方法:采用高效液相色谱法进行测定,色谱柱为十八烷基硅烷键合硅胶为填充剂(4.6 mm×250 mm,5μm),甲醇-水-磷酸(25∶75∶0.2)为流动相,检测波长250 nm。结果:葛根素在0.559 5～111.9 mg.L-1进样质量浓度与峰面积呈良好的线性关系(r=0.999 8,n=6),葛根素平均回收率为98.85%(n=6 RSD 0.83%);精密度试验(RSD 0.52%,重复性试验RSD 0.57%)。结论:方法快速、简便、准确、专属性强、重复性好,可测定柴芩软胶囊中葛根素的含量。
Objective：To establish a HPLC method for determining puerarin in Chaiqin soft capsules.Method： The HPLC analysis was performed on a C18 column（4.6 mm×250 mm,5 μm） with methanol-water-phosphoric acid（25 ∶ 75 ∶ 0.2） as mobile phase,detected at the wavelength of 250 nm.Result： Puerarinwas linear in the range of 0.559 5-111.9 mg · L-1（r=0.999 8）.The average recovery was 99.85%,RSD 0.83%.the RSDs of precision and reproducibility were 0.52% and 0.57% respectively.Conclusion： The method is simple,accurate and reproducible,which can be used to determine puerarin in Chaiqin soft capsules.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第18期98-100,共3页
Chinese Journal of Experimental Traditional Medical Formulae
