摘要
采用分光光度计法和实时荧光PCR分析茎点枯病菌诱导下芝麻过氧化物酶(POD)的活性变化和基因表达,以探讨POD在芝麻抗茎点枯病过程中的作用。酶活变化分析表明,在芝麻茎点枯病菌诱导下抗病品种豫芝11POD活性比感病品种冀9014升高快,感病品种在病原菌诱导36h后,POD活性才明显上升且高于抗病品种。定量PCR分析结果显示,在芝麻茎点枯病菌诱导后,抗病品种豫芝11中POD基因的表达从开始就有上调表达趋势,而感病品种冀9014在24h后才上调表达,且表达量前者高出后者5倍多。在芝麻茎点枯病菌诱导下,POD活性和基因的表达在芝麻抗病品种豫芝11和感病品种冀9014中有明显差异,可以推测,酶的活性变化、基因上调表达时间及表达量上的差异与芝麻的抗病反应有着密切关系。
POD activity and expression pattern induced by Macrophomina phaseolina were investi- gated respectively by spectrophotometer and real-time PCR,to elucidate the role of POD in sesa- me resistance against the stem rot. The measurement of POD activity suggested that POD activity in the resistant cultivar Yuzhi 11 increased faster than in the susceptible cultivar Ji 9014 induced by M. phaseolina. But after 36 hours, the enzyme activity dramatically increased in the susceptible cultivar,which was higher than in the resistant cultivar. The result of real-time PCR showed that the expression of POD gene promptly increased in the resistant cultivar Yuzhi 11 induced by the M. phaseolina,while the tendency in the susceptible cultivar Ji 9014 showed up-regulated until af- ter 24 hours. The expression level in the former was remarkably higher than in the latter （over 5 times）. The enzyme activity and the gene expression level of POD were observably different in the resistant cultivar Yuzhi 11 and the susceptible cultivar Ji 9014 induced by M. phaseolina. Based on the difference of enzyme activity and expression in quantity and time in different cultivars,POD was speculated to be involved in sesame defense response to M. phaseolina.
出处
《河南农业科学》
CSCD
北大核心
2012年第8期93-98,共6页
Journal of Henan Agricultural Sciences
基金
现代农业产业技术体系建设专项(CARS-15-1-05)
关键词
芝麻
茎点枯病菌
过氧化物酶
酶活
表达模式
抗病反应
Sesarnum indicum
Macrophomina phaseolina
peroxidase
enzyme activity
expres- sion pattern
disease-resistant response
