摘要
测定了接种溃疡病菌(Botryosphaeria dothidea)后两种抗性水平不同的杨树(Populus spp.)树皮中超氧阴离子(O2.-)、过氧化氢(H2O2)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)及膜脂过氧化产物丙二醛(MDA)含量的动态变化,分析了杨树与溃疡病菌互作中活性氧(Reactive Oxygen Species,ROS)的产生、抗氧化酶活性及膜脂过氧化水平的变化规律及与杨树抗病性的关系。结果表明,随接种时间的延长,两种杨树O2.-产生速率和H2O2含量均有上升趋势,抗病杨树毛白杨(P. tomentosa Carr.)出现了较明显的"氧化爆发",感病杨树北京杨(P. beijingensis Hsu)则不明显;POD活性持续上升,毛白杨的上升幅度大于北京杨的;SOD、CAT活性先上升后下降,北京杨两种酶活性在后期受到的抑制更明显;APX活性则在两种杨树中变化较大,毛白杨的变幅明显比北京杨的大;MDA含量增加,表明"氧化爆发"启动了膜脂过氧化,北京杨在接种后期MDA含量大幅上升,并且MDA含量与O2.-产生速率呈显著正相关。
The dynamic changes of superoxide anion O2^.- , hydrogen peroxide （H2O2） , superoxide dismutase （SOD）, catalase （CAT）, peroxidase （POD）, ascorbate peroxidase （APX） and malondialdehyde （MDA） in the barks of resistant and susceptible poplar cultivars, Populus tomentosa and P. beifingensis infected with Botryosphaeria dothidea, were measured, and the generation of reactive oxygen species （ROS） in the interaction between poplar cuhivars and Botryosphaeria pathogen, the activity change rule of oxidation enzyme-resistance and their relationship with the disease resistance of testing poplars were also analyzed in the paper. The results showed that the content of O2^.- and H2O2 in both poplar cuhivars increased with the time after inoculation. The ＂ oxidative burst＂ was clear in resistant cultivar and unclear in susceptible one; POD activity increased with a higher level in resistant cultivar than that in the susceptible one at the late stage; SOD and CAT activities increased at first stage and declined later, while the inhibition was more significant in the susceptible one; APX activity changed a lot in two cultivars and variable range was significant in the resistant one. The increased MDA appeared that ＂oxidative burst＂ was likely to initiate membrane lipid peroxidation. MDA content has a sharp increase in testing P. beijingensis at late stage after inoculation and a significant positive correlation with the rate of O2^.- generation.
出处
《南京林业大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第5期41-46,共6页
Journal of Nanjing Forestry University：Natural Sciences Edition
基金
国家自然科学基金资助项目(30671682)
国家“十一五”科技支撑项目(2006BAD08A111)
关键词
杨树
溃疡病菌
活性氧
抗氧化酶
丙二醛
Populus spp.
Botryosphaeria dothidea
Reactive oxygen species
Defense enzyme
Malondialdehyde
